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Plant seed separation layer area developmental regulation gene and application thereof

A seed and delamination technology, applied in the fields of biotechnology and botany, can solve the problems of flower or fruit shedding, crop or fruit tree production reduction, etc.

Active Publication Date: 2013-09-11
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a series of environmental factors, such as water shortage, extreme temperature, or the invasion of insects and pathogenic bacteria, etc. can also cause early abscission of leaves, flowers or fruits (Taghizadeh, M.S. et al. (2009). Water deficit changes the anatomy of the fruit abscission zone in Raphanus raphanistrum (Brassicaceae). Aust. J. Bio. 57: 708-714)
Untimely shedding can result in reduced yields for many crops or fruit trees

Method used

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  • Plant seed separation layer area developmental regulation gene and application thereof
  • Plant seed separation layer area developmental regulation gene and application thereof
  • Plant seed separation layer area developmental regulation gene and application thereof

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Experimental program
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Effect test

Embodiment approach

[0153] As an embodiment of the present invention, the gene encoding the SHAT1 protein is cloned into an appropriate vector by conventional methods, and the recombinant vector with the foreign gene is introduced into the plant cells that can express the SHAT1 protein , making the plant cell express SHAT1 protein. Plants overexpressing SHAT1 protein can be obtained by regenerating said plant cells into plants.

[0154] Preferably, a method for suppressing gramineous seed shattering comprises:

[0155] (s1) providing an Agrobacterium carrying an expression vector, the expression vector containing an interfering molecule of the SHAT1 gene (including but not limited to dsRNA, antisense nucleic acid, small interfering RNA, microRNA, or capable of expressing or forming the dsRNA, anti constructs of sense nucleic acid, small interfering RNA, microRNA);

[0156] (s2) contacting the plant cell, tissue or organ with the Agrobacterium in step (s1), so that the interfering molecule of th...

Embodiment 1

[0255] Example 1, isolation and phenotypic identification of shat1 and shat2 mutants

[0256] In order to study the grain shattering pathway, the inventors used the replacement line SL4 as the starting material, its fourth coloring was replaced by wild rice W1943, and the rest of the genetic background was indica rice Guangluai 4 (GLA4) ( figure 1 A-B).

[0257] SL4 exhibited extremely strong seed-shattering ability, which was due to the SH4 gene from wild rice on the one hand, and the seed-shattering gene qSH1 ( figure 2 A, WT). The inventors carried out cobalt 60 radioactive mutagenesis on SL4, from T 1 Plants without shattering were screened in the generation. The present inventor has isolated two extreme non-shattering mutants, named respectively as shattering abortion 1 (shat1) ( figure 2 A, second from left) and shattering abortion 2 (shat2) ( figure 2 A, third from left).

[0258] In addition to not shattering, shat1 also exhibited a series of abnormal floral o...

Embodiment 2

[0259] Embodiment 2, the disappearance of abscission layer is the reason that causes shat1 and shat2 mutants not to shatter

[0260] The non-shattering traits of shat1 and shat2 mutants were further detected by the Breaking Tensile Strength (BTS) tensile value at different developmental stages of seeds. The BTS pulling force value is negatively correlated with the seed shattering property, that is, the larger the pulling force value is, the harder it is for the seeds to shatter. During the first 6 days of flowering, there were no significant changes in the BTS values ​​of different rice varieties. In wild-type SL4, pull force values ​​decreased rapidly on day 11 after anthesis. On the 13th day after flowering, the BTS value was difficult to detect because the connection between the seed and the small branch was already very weak. On the 15th day after flowering, most of the wild-type seeds had fallen off automatically ( figure 2B). These changes were mainly caused by the ...

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Abstract

The invention relates to a plant seed separation layer area developmental regulation gene and an application thereof, and discloses a gene for effectively regulating the shattering performance of the grass family, i.e. the SHAT1 gene. The SHAT1 gene can regulate the plant separation layer area cell development, so that the shattering performance of plants is improved. The gene can be applied to plant cross breeding so as to obtain the plants with improved shattering performance or improved breeds.

Description

technical field [0001] The invention belongs to the fields of biotechnology and botany; more specifically, the invention relates to a gene for regulating the development of a plant seed abscission zone and application thereof. Background technique [0002] Although shattering is beneficial to seed dispersal in wild rice, it often brings negative effects such as yield reduction in cultivated rice. Human ancestors began the process of domesticating rice from the perspective of selection to reduce grain shattering. The shedding of rice seeds occurs in a place known as the disarticulation zone (abscission zone). This area consists of one or two layers of cells that are small in shape, dense in cytoplasm, and arranged in a flat shape. These cells show different physiological and biochemical responses from surrounding cells in response to some biological or non-biological signals that promote shedding (Hoekstra, G.J. et al. (2001). Anatomical features at the disarticulation zone...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
Inventor 韩斌周艳吕丹凤李灿阳罗江虹朱静洁上官颖颖
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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