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Method for separating and purifying three flavonoid glycosides from stems and leaves of trichosanthes kirilowii

A technology for separation and purification of flavonoid glycosides, applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve problems such as waste disposal, waste of resources, and insufficient utilization of stems and leaves, and achieve purity and Improve efficiency, prolong service life, and save reagents

Inactive Publication Date: 2013-09-11
LIAOCHENG UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the stems and leaves of cucurbit plants have not been fully utilized at present, and most of them are discarded as waste, resulting in a great waste of resources.

Method used

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  • Method for separating and purifying three flavonoid glycosides from stems and leaves of trichosanthes kirilowii
  • Method for separating and purifying three flavonoid glycosides from stems and leaves of trichosanthes kirilowii
  • Method for separating and purifying three flavonoid glycosides from stems and leaves of trichosanthes kirilowii

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Embodiment

[0030] Example: The method for isolating and purifying flavonoid glycosides from stems and leaves of Trichosanthes chinensis, the steps of which are:

[0031] (1) Preparation of the crude extract from the stems and leaves of Trichosanthes chinensis: after crushing the stems and leaves of Trichosanthes chinensis, heat and reflux extraction with 70% ethanol as solvent for 4 times, combine the extracts, and concentrate under reduced pressure to obtain the crude extract.

[0032] (2) Extraction: Disperse the crude extract of the stems and leaves of Trichosanthes chinensis in water, extract with equal volumes of petroleum ether and ethyl acetate successively, and concentrate the petroleum ether and ethyl acetate layers under reduced pressure respectively to obtain the petroleum ether fraction and ethyl acetate parts.

[0033] (3) Separation and purification by semi-preparative high-performance liquid chromatography: the ethyl acetate part of the stems and leaves of Trichosanthes ...

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Abstract

The invention relates to a method for separating and purifying three flavonoid glycoside type components from stems and leaves of trichosanthes kirilowii. The stems and the leaves of the trichosanthes kirilowii are taken as raw materials, and the method comprises the following steps: (1) preparing a crude extract of the stems and the leaves of the trichosanthes kirilowii; (2) extracting; (3) performing semi-preparation type high performance liquid chromatography separation and purification: performing separation and purification on an ethyl acetate part by semi-preparation type high performance liquid chromatography, wherein a mobile phase is methanol-water, and three high-purity flavonoid glycoside type components, namely luteolin-7-O-beta-glucoside, apigenin-7-O-beta-glucoside and chrysoeriol-7-O-beta-glucoside are respectively obtained. The process flow is green and environment-friendly, has no serious harm to an environment and is low in comprehensive cost.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method of separating and purifying three kinds of flavonoid glycosides (luteolin-7-O-β-glucoside, apigenin-7-O-β-glucoside and cotyledon Ellisin-7-O-β-glucoside) method. Background technique [0002] Cucurbitaceae Trichosanthes genus Trichosanthes ( Trichosanthes kirilowii Marim.) and Bilateral Trichosanthes ( Trichosanthes rosthorinii Harms) is the original plant of the Trichosanthes medicinal material stipulated in the Chinese Pharmacopoeia, and its fruit, peel, seeds and tubers are used as Trichosanthes, Trichosanthes skin, Trichosanthes seeds and trichosanthes respectively. The stems and leaves of Trichosanthes chinensis are sour, cold in nature, non-toxic, and return to the liver and spleen meridians. They have the effect of clearing away heat and relieving summer heat. Ascites-type cancer cells in the dead body have anti-cancer effects. [0003] There a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 李爱峰张永清柳仁民孙爱玲
Owner LIAOCHENG UNIV
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