Method for determining contents of cystine, cysteine and salt thereof in amino acid injection

Abstract

The invention relates to a method for determining contents of cystine, cysteine and salt thereof in amino acid injection. The method comprises the following steps: measuring a sample to be detected and a reference solution precisely, adding 0.1-1% of sodium pyrosulfite solution, shaking up, heating by 70-100 DEG C. for 10-60 minutes, fetching out, adding formic acid 1.5 ml and 30% superoxol 1.0 ml, shaking up, placing in 30+-2 DEG C. for 30 minutes, adding water to the scale, shaking up, fetching the sample to be tested and injecting into a chromatograph with cation exchange resin, carrying out gradient elution and regeneration balance by a special-purpose mobile phase A6 solution and a special-purpose sodium hydroxide regeneration liquid, carrying out a post-column derivatization reaction by a special-purpose derivatization reagent ninhydrin solution, recording a chromatogram with the detection wavelength 570 nm, and obtaining the contents by calculating peak areas according to external standard method. The invention has the beneficial effects that (1) short analysis time and saved detection time; (2) high sensitivity; (3) low price and saved detection cost; (4) good specialization.

Description

technical field [0001] The invention relates to a method for determining the content of cystine, cysteine ​​and salts thereof, in particular to a method for determining the content of cystine, cysteine ​​and salts thereof in amino acid injection. Background technique [0002] Cystine is a non-essential amino acid in the human body, and its increased concentration in the body is considered to be the initiating factor of atherosclerosis. Among them, homocysteine ​​is associated with cardiovascular diseases (coronary artery disease, hypertension), nerve It is closely related to the occurrence of systemic diseases (stroke), diabetes, kidney disease, pregnancy-related diseases, and Alzheimer's disease. In the late 1960s, some scholars noticed that most patients with hereditary homocystinuria had subclinical cardiovascular disease. In 1976, Wichen et al. proposed through epidemiological investigation that homocysteine ​​is an independent risk factor for cardiovascular patients, b...

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Problems solved by technology

[0003] At present, the method for analyzing the contents of cystine, cysteine ​​and their salts in amino acid nutritional injections is generally the waters derivatization method, which has the following disadvantages that are difficult to overcome: 1) This method uses a special AccQ-FluorTM reagent , due to its monopoly position, the reagent price is relatively expensive; 2) In the process of collecting cystine, cysteine ​​and its salts during analysis, other amino acids are also collected at the same time, resulting in many unknown peaks in the analysis process, Other collected substances interfere with the content analysis of cystine, cysteine ​​and their salts; 3) Other collected substances also affect the sensitivity of the analysis

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