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Method for producing yeast cells by fermenting protein and impurity removed soybean liquid

A technology of yeast cells and bean water, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as environmental pollution, reduction, resource waste, etc., to solve resource waste and environmental pollution problems, reduce production cost, ease of handling

Inactive Publication Date: 2013-09-04
SHANDONG GAOTANG LANSHAN GRP CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soy clear water also contains a large amount of low-molecular protein, carbohydrates, peptides and other substances. Using this method, soy clear water is used to produce yeast cells. The total protein yield of a single yeast strain is 2.42g / L, and the COD is significantly reduced. The COD removal rate reaches 40%, which greatly solves the problem of waste of resources and environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The selected yeast strain is Candida tropicalis.

[0026] A kind of soya bean clear water fermentation method step of producing yeast cell of the present invention is:

[0027] (1) Treatment of soybean clear water: heat soybean clear water at 120°C for 10 minutes to flocculate and precipitate part of the protein, and then let it stand for 40 minutes to obtain the supernatant;

[0028] (2) Strain activation: sterilize the supernatant at 0.1Mpa, 121°C for 15 minutes, then insert yeast strains at a volume ratio of 2‰ after cooling, and incubate at 28°C, 120r / min for 16 hours;

[0029] (3) Fermentation: Sterilize the supernatant at 0.1Mpa, 121°C for 15 minutes, add activated strains at a volume ratio of 1:19 for the activated strains to the supernatant after cooling, and heat at 28°C, 120r / h Min shaker culture 24h.

Embodiment 2

[0031] The selected yeast strain is Saccharomyces cerevisiae.

[0032] A kind of soya bean clear water fermentation method step of producing yeast cell of the present invention is:

[0033] (1) Treatment of bean water: heat bean water at 110°C for 20 minutes to flocculate and precipitate part of the protein, and then let stand for 35 minutes to obtain the supernatant;

[0034] (2) Strain activation: sterilize the supernatant at 0.1Mpa, 121°C for 30 minutes, then insert yeast strains at a volume ratio of 3‰ after cooling, and incubate at 30°C, 100r / min for 16 hours;

[0035] (3) Fermentation: Sterilize the supernatant at 0.1Mpa, 121°C for 30 minutes, add activated strains at a volume ratio of 1:25 to the supernatant after cooling, and add activated strains at 30°C, 100r / Min shaker culture 26h.

Embodiment 3

[0037] The selected yeast strain is Saccharomyces karlsberg.

[0038] A kind of soya bean clear water fermentation method step of producing yeast cell of the present invention is:

[0039] (1) Treatment of bean water: heat bean water at 115°C for 20 minutes to flocculate and precipitate part of the protein, and then let it stand for 30 minutes to obtain the supernatant;

[0040] (2) Strain activation: sterilize the supernatant at 0.1Mpa, 121°C for 20 minutes, after cooling, insert yeast strains at a volume ratio of 10‰, and culture at 37°C for 10 hours;

[0041] (3) Fermentation: Sterilize the supernatant at 0.1Mpa, 121°C for 20 minutes, add activated strains according to the volume ratio of the activated strains to the supernatant of 1:15 after cooling, and stand at 37°C Cultivate for 18h.

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PUM

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Abstract

The invention provides a method for producing yeast cells by fermenting a protein and impurity removed soybean liquid. A liquid left after separating soybean proteins from degreased soybeans and centrifuging for removing impurities is called as the protein and impurity removed soybean liquid. The protein and impurity removed soybean liquid also contains large amounts of low-molecular-weight proteins, carbohydrates, peptides and the like, and the method for producing the yeast cells by fermenting the protein and impurity removed soybean liquid allows the total protein yield of a single yeast to be 2.42g / L, the COD to be obviously reduced, the COD removal rate to reach 40%, and substantially solves resource waste and environmental pollution problems.

Description

technical field [0001] The invention relates to a method for fermenting and producing yeast cells, in particular to soybean clear water and a method for fermenting and producing yeast cells. Background technique [0002] Soybean is the main source of vegetable protein and edible oil, and it is also the most productive oil crop in the world. The nutrients contained in soybeans are relatively comprehensive and rich in content. Every 100 grams of soybeans contain 36.3 grams of protein, 18.4 grams of fat, 25.3 grams of sugar, 412 kcal of calories, 367 mg of calcium, 571 mg of phosphorus, 11 mg of iron, and 0.4 mg of carotene. Vitamin B1 0.79mg, Vitamin B2 0.25mg, Niacin 2.1mg. Soy contains various amino acids needed by the human body, especially lysine, leucine, threonine and other essential helium acids. Soybean contains a variety of unsaturated fatty acids, especially linolenic acid is the most abundant. This has a great effect on preventing arteriosclerosis, and soybeans a...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/18C12R1/865C12R1/74C12R1/86
Inventor 许兰山许振国殷燕穆洪静高剑
Owner SHANDONG GAOTANG LANSHAN GRP CORP
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