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Method for obtaining dwarfed early gold sweet orange regeneration plant through agrobacterium rhizogenes

A technology of Agrobacterium rhizogenes and regenerated plants, which is applied in botany equipment and methods, plant regeneration, horticultural methods, etc. It can solve the problems affecting the enthusiasm of fruit farmers for production and the increase of labor costs in citrus production, so as to achieve easy survival, easy rooting, Obvious effect of dwarfing

Inactive Publication Date: 2014-06-25
HUAZHONG AGRI UNIV
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Problems solved by technology

However, with the development of urbanization in my country, a large number of rural labor force has been transferred, and the labor cost of citrus production has increased, which has affected the enthusiasm of fruit farmers; in addition, the laborers in orange orchards are aging, and only the development of a simplified cultivation model is suitable for the development of the rural citrus industry.

Method used

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  • Method for obtaining dwarfed early gold sweet orange regeneration plant through agrobacterium rhizogenes
  • Method for obtaining dwarfed early gold sweet orange regeneration plant through agrobacterium rhizogenes
  • Method for obtaining dwarfed early gold sweet orange regeneration plant through agrobacterium rhizogenes

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Embodiment 1

[0033] Embodiment 1: a kind of method utilizing Agrobacterium rhizogenes to obtain dwarf early golden sweet orange regeneration plant, comprises the following steps:

[0034] 1. Explant preparation:

[0035] Early golden sweet orange fruits were collected from the Citrus Breeding Center of Huazhong Agricultural University. Take out the seeds from the citrus fruit, rinse them with tap water, soak them in 1M NaOH solution for 10 minutes, remove the pectin, and rinse them with distilled water; soak them in 3% (v / v) sodium hypochlorite solution on an ultra-clean bench for 20 minutes, and wash them with sterilized distilled water for 3 times , 5 minutes each time; the sterilized seeds were sown on the MT seeding medium after peeling off the inner and outer seed coats with a sterile scalpel and tweezers on the ultra-clean workbench. , after turning green for a while, select robust aseptic seedlings and cut the epicotyls into about 1.5cm for later use;

[0036] 2. Preparation of Ag...

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Abstract

The invention discloses a method for obtaining dwarfed early gold sweet orange regeneration plant through agrobacterium rhizogenes. According to the characteristic of dwarfed plant type of plant transformed and regenerated through the agrobacterium rhizogenes, polyembryony breed of early gold sweet orange is transformed through agrobacterium rhizogenes NSU440, hairy roots are induced, then complete plant is obtained through a way of germination of hairy roots and radication of regeneration buds, and finally a dwarfed breed suitable for labor-saving cultivation is cultivated, so that the fruit garden labor efficiency is improved, and the labor expenditure is reduced. Transformation receptor material adopts early gold sweet orange aseptic seedling epicotyl, and as sweet orange belongs to a polyembryony breed, regeneration plant can keep parental excellent quality.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for obtaining dwarf early golden sweet orange regeneration plants by using Agrobacterium rhizogenes. Background technique [0002] Citrus is an important fruit tree in the world. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), in 2009, the planting area of ​​citrus in the world reached 8.719 million hectares, with a total output of about 124 million tons; in the same period, the planting area of ​​citrus in my country was about 2 million hectares, and the output reached 25 million tons. Both rank first in the world. However, with the development of urbanization in my country, a large number of rural laborers have transferred, and the labor cost of citrus production has increased, which has affected the enthusiasm of fruit farmers. In addition, the workers in orange orchards are aging. Only the development of a simp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H4/00
Inventor 郭文武肖璇陈春丽
Owner HUAZHONG AGRI UNIV
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