Application of fibroblast activation protein (FAP) alpha to preparation of pancreatic cancer prognosis kit

A technology for activating proteins and fibroblasts, which is used in medicine, the diagnosis and treatment of pancreatic cancer, and can solve problems such as no in-depth research.

Active Publication Date: 2013-08-28
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the role and mechanism of pancreatic stellate cells with different phenotypes in the progression of pancreatic cancer have not been studied in depth

Method used

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  • Application of fibroblast activation protein (FAP) alpha to preparation of pancreatic cancer prognosis kit
  • Application of fibroblast activation protein (FAP) alpha to preparation of pancreatic cancer prognosis kit
  • Application of fibroblast activation protein (FAP) alpha to preparation of pancreatic cancer prognosis kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Correlation between fibroblast activation protein in the stroma of pancreatic cancer and the clinicopathological characteristics and prognosis of pancreatic cancer

[0045] 1. Subjects

[0046] Pathological tissue samples were obtained from Peking Union Medical College Hospital, Chinese Academy of Medical Sciences. Sample inclusion and exclusion criteria are as follows:

[0047] (1) Inclusion criteria: cases undergoing surgical resection, and postoperative pathological diagnosis of pancreatic ductal adenocarcinoma;

[0048] (2) Exclusion criteria: incomplete case data, no pathology and follow-up data; tumors were not resected, or tumor biopsy was performed only, and complete histological specimens were not obtained; no corresponding paraffin specimens were found in the pathological specimen bank; paraffin specimens Sections for immunohistochemistry could not be prepared.

[0049] In this example, 56 pancreatic cancer patients were included, including 24 ma...

Embodiment 2

[0077] Example 2 Pancreatic cancer cell culture and passage

[0078] 1. Materials

[0079] (1) The human pancreatic cancer cell lines BxPC3, Capan1 and PANC1 were donated by Professor Freiss.H of the Technical University of Munich, Germany.

[0080] The surgically resected non-tumor pancreatic tissues of pancreatic cancer patients hospitalized for basic surgery in Peking Union Medical College Hospital were collected, and primary human pancreatic stellate cells were obtained, marked with PSC1#, PSC2#, ...PSCn#.

[0081] (2) Consumables, reagents and instruments related to cell culture

[0082] Reagent / consumable name

manufacturers

Cell culture flasks, cell freezing tubes, centrifuge tubes

Corning

Disposable pipette

Costar

Optical microscope

COIC

Electric Auxiliary Pipette D03923

Accu-JetPro

High Pressure Steam Cooker MLS3750

SANYO

C02 Cell Incubator MCO-15AC

SANYO

Cell counter NeucleoCounter...

Embodiment 3

[0095] Example 3. Identification of primary human pancreatic stellate cells and expression of FAPα

[0096] 1. Identification of human primary pancreatic stellate cells and the expression of FAPα by immunofluorescence

[0097] (1) Relevant consumables, reagents and instruments

[0098] Table 4. Relevant consumables, reagents and instruments required for immunofluorescence detection

[0099]

[0100] (2) method

[0101] Human primary pancreatic stellate cells were planted on Nunc Lab-Tek Ⅱ chamber coverslips, respectively, with serum-free DMEM / F12 medium, DMEM / F12 medium + TGFβ1 (10ng / ml), pancreatic cancer cell BxPC3 , Capan1 and PANC1 conditioned medium culture, pancreatic cancer cells BxPC3, Capan1 and PANC1 conditioned medium culture + TGFβ neutralizing antibody, when the pancreatic stellate cells confluence reached 80-90%, perform immunofluorescence detection; remove the culture Base, add 100 μl formalin solution to each well, fix for 30 minutes; suck out the formali...

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PUM

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Abstract

The invention relates to an application of fibroblast activation protein (FAP) alpha to the preparation of a pancreatic cancer prognosis kit and an application of an FAP alpha inhibitor to the preparation of drugs for treating pancreatic cancer. The high expression of the FAP alpha in pancreatic cancer tissue mesenchyme of a testee has shorter overall survival than low expression. The FAP alpha inhibitor can be used for inhibiting the effects of FAP alpha (+) pancreatic stellate cells on promoting the pancreatic cancer migration, invasion and metastasis in vivo, which hints that the FAP alpha inhibitor can be used for inhibiting the metastasis of the pancreatic cancer and decelerating the progress of pancreatic cancer; and aiming at the pancreatic cancer mesenchyme treating strategy, the comprehensive treatment effect for the pancreatic cancer is possibly improved.

Description

technical field [0001] The present invention relates to the field of medicine. More specifically, the field of diagnosis and treatment of pancreatic cancer. In particular, it relates to the use of fibroblast activation protein alpha in diagnosing the prognosis of pancreatic cancer, and the use of fibroblast activation protein alpha inhibitors in the treatment of pancreatic cancer. Background technique [0002] Pancreatic cancer is a malignant tumor with rapid progress and high mortality. Its remarkable pathological features are more stroma components and obvious fibroproliferative reaction. Recently, the microenvironment surrounding pancreatic cancer cells has received increasing attention. The pancreatic cancer microenvironment is characterized by a pronounced desmoplastic response of tumor stromal components [1]. The stroma of pancreatic cancer is a dynamic environment in which pancreatic stellate cells (PSC), extracellular matrix, matrix metalloproteinases, matrix meta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/574A61K45/00A61K31/40A61P35/00
Inventor 赵玉沛文张廖泉张太平戴梦华胡亚由磊周立舒红徐强赵建国韦颍昕牛哲禹刘乔飞
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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