Method for extracting plant active substance capable of inhibiting bacterial wilt of tobacco

A tobacco bacterial wilt bacteria and inhibition technology are applied in the directions of botanical equipment and methods, plant growth regulators, chemicals used for biological control, etc., to achieve the effects of convenient operation, simple extraction method, and easy promotion and application.

Active Publication Date: 2013-08-28
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method for extracting antibacterial substances from amaranth leaves has not been reported. Based on this point, the inventor has carried out a study on the extraction method of antibacterial substances in amaranth leaves, aiming to provide a theoretical basis for developing new botanical pesticides

Method used

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  • Method for extracting plant active substance capable of inhibiting bacterial wilt of tobacco

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Experimental program
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Effect test

Embodiment 1

[0018] Amaranth leaves were washed and dried in the sun, and pulverized by a high-speed pulverizer at a speed of 12000r / min for 3 minutes to a fine powder of 100-120 mesh to obtain amaranth leaf powder. Take 10 g of amaranth leaf powder and put it into a conical flask, extract the amaranth leaf powder with analytically pure ethyl acetate, add ethyl acetate in 3 times, add 100 mL each time, put the conical flask on a shaker at 20°C Shake at 90r / min for 36 hours, filter, collect the filtrate, combine the filtrates for 3 times, put the filtrate into a rotary concentration evaporator, under the conditions of 0.09MPa pressure, water bath temperature of 40-43°C and condensation temperature of 8-10°C, 80r / min evaporated and concentrated to a paste to obtain a black extract.

Embodiment 2

[0020] Amaranth leaves are washed and dried in the sun, and pulverized by a high-speed pulverizer at a speed of 8000r / min for 6 minutes to a fine powder of 100-120 mesh to obtain amaranth leaf powder. Take 10 g of amaranth leaf powder and put it into a conical flask, extract the amaranth leaf powder with analytically pure ethyl acetate, add ethyl acetate in 3 times, add 200mL each time, put the conical flask on a shaker at 25°C Shake at 120r / min for 24 hours, filter, collect the filtrate, combine the filtrates for 3 times, put the filtrate into a rotary concentration evaporator, under the conditions of 0.1MPa pressure, water bath temperature of 40-43°C and condensation temperature of 8-10°C, 120r / min evaporated and concentrated to a paste to obtain a black extract.

Embodiment 3

[0022] Amaranth leaves are washed and dried in the sun, and pulverized by a high-speed pulverizer at a speed of 10,000 r / min for 5 minutes to a fine powder of 100-120 mesh to obtain amaranth leaf powder. Take 10g of amaranth leaf powder and put it into a conical flask, extract the amaranth leaf powder with analytically pure ethyl acetate, add ethyl acetate in 3 times, add 150mL each time, put the conical flask on a shaker at 22°C Shake at 100r / min for 32h, filter, collect the filtrate, combine the filtrate for 3 times, put the filtrate into a rotary concentration evaporator, under the conditions of 0.1MPa air pressure, water bath temperature of 40-43°C and condensation temperature of 8-10°C, 90r / min evaporated and concentrated to a paste to obtain a black extract.

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Abstract

The invention belongs to the technical field of biopesticides, and particularly provides a method for extracting a plant active substance capable of inhibiting bacterial wilt of tobacco. According to the method, amaranth leaves, taken as the raw material, are smashed after being dried, the amaranth leaf powder is extracted by analytically pure ethyl acetate, the collected extracting solution is put into a rotary concentration evaporator to be concentrated to form paste, and then the antibacterial substance is obtained. The method is simple, fast, convenient to operate, and easy for promotion and application.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to an extraction method of a plant active substance that has an inhibitory effect on R. solanacearum, more specifically, a method for extracting an antibacterial active substance from amaranth leaves. Background technique [0002] In agricultural production, chemical pesticides to control plant pests are widely used, such as insecticides, fungicides, herbicides, etc. Chemical pesticides play an important role in increasing food production. Although chemical pesticides have the advantages of quick-acting, easy processing, and obvious effects of controlling harmful organisms, due to a series of problems such as polluting the environment, destroying ecological balance, pesticide residues, and bacterial resistance, it is necessary to explore new control methods. [0003] At present, the use of biological control is not only safe, efficient, low-toxic, and non-pollutin...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P3/00
Inventor 廖晓兰张亚苏品黄璜刘双清夏日照仇佳伟尹伟
Owner HUNAN AGRICULTURAL UNIV
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