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Treatment and prevention of malaria

一种疟疾、序列的技术，应用在治疗和预防疟疾领域，能够解决抗体诱导弱效力等问题

Inactive Publication Date: 2013-08-21
WALTER & ELIZA HALL INST OF MEDICAL RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, DNA vaccines have demonstrated weak potency regarding antibody induction in humans (Wang et al., 2001)

Method used

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  • Treatment and prevention of malaria
  • Treatment and prevention of malaria
  • Treatment and prevention of malaria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0356] Example 1. Identification of pfRip as a Rh5 complex partner

[0357] Purification of processed 45 kDa pfRh5 from parasite culture supernatants by ion exchange chromatography. Analysis of pfRH5 by size exclusion chromatography on a Superdex 200 analytical column confirmed that pfRH5 elutes as a ~150-200 kDa species ( figure 1 A). Blue native gel electrophoresis confirms that pfRh5 migrates on the gel as a ~150-200kDa species ( figure 1 B).

[0358] To determine whether pfRh5 complexes with other molecules or forms homo-oligomers, proteins were incubated with pfRh5 antibodies and analyzed by size exclusion chromatography. The 300 μl pfRh5-containing fraction separated from the culture supernatant was loaded onto a Superdex200 analytical column and eluted with PBS ( figure 2 A). The same 300 μl sample was pre-incubated with 25 μg monoclonal pfRh5 antibody for 15 minutes at room temperature and then 2 hours on ice before loading onto a Superdex200 analytical column ...

Embodiment 2

[0362] Example 2.pfRip falls off in the culture supernatant

[0363]A single Strep tag and a triple hemagglutinin (HA) tag were added to the C-terminus of pfRip by 3′-single homologous exchange recombination ( image 3 A). Western blotting of saponin clumps and HA-tagged proteins purified from the culture supernatant of the pfRip HA line with an anti-HA antibody confirmed that PfRip was processed and shed into the culture supernatant ( image 3 B). PfRipHA was analyzed by SDS-PAGE under reducing and non-reducing conditions and transferred to nitrocellulose membranes. Immunoblot with anti-HA antibody showed that the processed C-terminal fragment migrated similarly under reducing and non-reducing conditions, implying that the N-terminus and C-terminus of pfRip are not linked by any disulfide bond after processing ( image 3 C).

Embodiment 3

[0364] Example 3. Immunoprecipitation of pfRip

[0365] Culture supernatants from wt3D7 and 3D7-pfRipHA parasite lines were immunoprecipitated with anti-HA-Sepharose beads. Bound material was separated by SDS-PAGE and transferred to nitrocellulose membranes to probe for pfRh5 (clone 2F1 ) using a monoclonal anti-pfRh5 antibody. Detection of pfRh5 in bound material from only the 3D7-pfRipHA line indicated that pfRh5 specifically co-immunoprecipitated with pfRipHA ( Figure 4 A).

[0366] Culture supernatants from wt3D7 and 3D7-pfRipHA parasite lines were immunoprecipitated with monoclonal anti-pfRh5 antibody coupled to microbeads, and culture supernatants from 3D7-pfRipHA parasites were incubated with microbeads alone as additional controls . Bound material was separated by SDS-PAGE and transferred to nitrocellulose membranes to probe pfRipHA using an anti-HA antibody ( Figure 4 B). Detection of pfRipHA in bound material from only the 3D7-pfRipHA parasite line immunopre...

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Abstract

The present invention relates to polypeptides from Plasmodium and polynucleotides encoding the polypeptides. The invention further relates to compositions comprising the polypeptides and their use in the treatment and prevention of malaria.

Description

field of invention [0001] The present invention relates to polypeptides from Plasmodium and polynucleotides encoding the polypeptides. The invention further relates to compositions comprising polypeptides and their use in the treatment and prevention of malaria. Background of the invention [0002] Human malaria is caused by infection with protozoan parasites of the genus Plasmodium. Four species are known to cause disease in humans: Plasmodium falciparum, Plasmodium malariae, Plasmodium ovale, and Plasmodium vivax. However, P. falciparum is responsible for most severe disease and death. Recent estimates of the annual number of clinical malaria cases worldwide range from 214,000,000 to 397,000,000 (The world health report 2002: reducing risks, promoting healthy life. Geneva: World Health Organization; Breman et al., 2004), and have been thought to be 515,000,000 in 2002 ( Range 300,000,000-660,000,000) higher estimates of clinical cases of P. falciparum (Snow et al., 2004...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/002A61P33/06G01N33/53A61K39/015C07K14/445G01N33/566
CPCG01N33/56905G01N2333/445G01N2500/02A61K39/015A61K2039/505A61P33/06Y02A50/30A61K39/002C07K14/445G01N33/53G01N33/566
Inventor 陈琳A·寇曼T·崔格利亚
Owner WALTER & ELIZA HALL INST OF MEDICAL RES
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