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Electrode modification method for adopting nucleic acid aptamers for high sensitivity detection of tumor cells

A nucleic acid aptamer and tumor cell technology, which is applied in the electrode modification field of nucleic acid aptamer for highly sensitive detection of tumor cells, can solve the problems of high cost, difficult to popularize and use, lack of tumor cell-specific antibodies, etc. Limits of detection, increased sensitivity, reduced background effects

Inactive Publication Date: 2013-08-21
SUZHOU UNIV
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Problems solved by technology

But the disadvantage is the lack of tumor cell-specific antibodies
However, the cost of this equipment is relatively high, and it is not easy to promote and use

Method used

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  • Electrode modification method for adopting nucleic acid aptamers for high sensitivity detection of tumor cells
  • Electrode modification method for adopting nucleic acid aptamers for high sensitivity detection of tumor cells
  • Electrode modification method for adopting nucleic acid aptamers for high sensitivity detection of tumor cells

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Embodiment Construction

[0034] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.

[0035] refer to figure 1 As shown, a nucleic acid aptamer is used for the electrode modification method of highly sensitive detection of tumor cells, comprising the following steps:

[0036] Step 1) Modification of the electrode surface

[0037] The glassy carbon electrode was polished with 1μm, 0.05μm Al2O3 powder in turn to remove surface impurities, and then respectively heated in 1:1HNO 3 , acetone, and double-distilled water for 2 minutes; put the electrode at 0.085mol / L K 2 Cr 2 o 7 and 1.7mol / L HNO 3 In the solution, use 1.5V constant potential oxidation for 30s to generate surface carboxyl groups; immerse the oxidized glassy carbon electrode in 0.02mol / L PBS (pH=6.8) solution containing 0.02mol / L EDC and 0.01mol / L NHS, at room temperature Activation for 30 minutes; 20μL 0.5*10 -5 mol / L 15T-TLS1c (TTTTTTTTTTTTTTTCAGGAG...

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Abstract

The invention discloses an electrode modification method for adopting nucleic acid aptamers for high sensitivity detection of tumor cells, wherein hepatic carcinoma cell BNL1MEA.7R.1(MEAR)-specific aptamers TLS1c and TLS11a are covalently modified on the surface of an electrode, and a cyclic voltammetry method, a differential pulse voltammetry method and an alternating current impedance method are adopted to detect MEAR cells, such that trace detection on the single MEAR cell can be achieved in 1 mL of 1*10<9> blood cells. With the technical scheme, a background value and a detection limitation can be significantly reduced, and sensitivity of electrochemical tumor cell detection can be improved.

Description

technical field [0001] The invention relates to an electrode modification method, in particular to an electrode modification method using a nucleic acid aptamer for highly sensitive detection of tumor cells. Background technique [0002] Tumors have always threatened human health. However, there is still no effective treatment. The main reason is that the early detection of tumors cannot be realized, which delays the best treatment time. Therefore, the development of a rapid, sensitive and accurate detection method is very important for the treatment of tumors. [0003] Circulating tumor cells (CTCs) refer to tumor cells that enter the peripheral blood circulation spontaneously or due to diagnostic and therapeutic procedures. They have high viability and high metastatic potential. They can survive in the circulation system and proliferate in a suitable environment, leading to tumor recurrence and metastasis. Tumor metastasis is the most insidious but deadly problem of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 彭睿曲丽明
Owner SUZHOU UNIV
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