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Method for preparing bacillus licheniformis

A Bacillus licheniformis, weight technology, applied in the field of fermentation engineering, can solve the problems to be improved, single product, etc., achieve the effects of improving gastrointestinal tract function, simple method, and convenient for industrialized implementation

Inactive Publication Date: 2015-04-22
XIAMEN LIUWEI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The product of the existing preparation method of bacillus licheniformis is single, and the current technology still needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: liquid fermentation

[0019] 1. Medium components: 0.5% by weight of starch, 0.5% by weight of urea, 1% by weight of soybean meal powder, 0.6% by weight of dipotassium hydrogen phosphate, 0.3% by weight of potassium dihydrogen phosphate, 0.05% by weight of yeast extract, and 0.1% by weight of magnesium sulfate , 0.02% by weight of manganese sulfate.

[0020] 2. Inoculation amount: 8% Bacillus licheniformis, from the School of Chemistry and Chemical Engineering of Xiamen University, preserved in CGMCC, preservation number CGMCC No.2876, preservation date January 14, 2009, patent number: ZL201010269454, publication date is December 01, 2010.

[0021] 3. Temperature: 37°C

[0022] 4. Initial ventilation volume: 1:0.5

[0023] 5. The pH value of the fermentation process: not less than 6.5

[0024] 6. Stirring speed: 250rpm

[0025] 7. The relative value of dissolved oxygen in the fermentation process: not less than 25%

[0026] 8. Filling pressure during ...

Embodiment 2

[0028] Example 2: solid fermentation

[0029] 1. Medium components: brown sugar 1% by weight, corn flour 50% by weight, bran 37.35% by weight, soybean meal 10% by weight, magnesium sulfate 0.6% by weight, ammonium sulfate 0.5% by weight, dipotassium hydrogen phosphate 0.5% by weight, Tween-80 0.05% by volume

[0030] 2. Inoculum size: 50% (using the fermented liquid obtained in Example 1)

[0031] 3. Fermentation method: closed anaerobic fermentation

[0032] 4. Fermentation process temperature: 35-38°C before 72 hours, and rise to 55-60°C after 72-96 hours

[0033] 5. Fermentation time: 96 hours

Embodiment 3

[0034] Example 3: Drying and testing

[0035] Low-temperature boiling drying is adopted, and the drying temperature is not higher than 85°C.

[0036] Example 1 uses a 1000-liter fermenter, the final output is 760 kg (weighed after direct drying), and the yield is 94.6%. (Yield rate = final product weight * 100% / (800+ liquid medium dry matter weight))

[0037] Detection method:

[0038] 1. Bacillus licheniformis: The total number of viable bacteria is detected by the method specified in NY1461-2007, the number of spores is detected by microscope counting, and the pH value is detected by a pH meter. The total number of live bacteria of Bacillus licheniformis is greater than or equal to 20 billion / g, and the number of spores is greater than or equal to 18 billion; the pH value is 5-6.

[0039] 2. Organic acids: Qualitative and quantitative detection with high performance liquid chromatography, the result is 0.50%, and the qualitative detection determines that the organic a...

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Abstract

The invention provides a method for preparing bacillus licheniformis. The method comprises the following steps of liquid state fermentation, solid fermentation, drying and detection of inoculated CGMCC No.2876 bacillus licheniformis. The bacillus licheniformis prepared by the method is accompanied by a plurality of other useful metabolites of organic acids, such as 3-methylbutyric acid, 2-methyl-butyric acid, phenylacetic acid, pentadecanoic acid, palmitic acid, linoleic acid and the like and digestive enzymes, such as amylase, protease and the like. A final product contains probiotics-bacillus licheniformis and prebiotics-metabolite, the gastroenteric function of animals can be obviously improved and the healthy growth of animals can be promoted due to the synthetic action of the two matters, and the effect is superior to that of a single viable organism. Meanwhile, the method is simple, low in cost and convenient in industrial implementation.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a preparation method of bacillus licheniformis. Background technique [0002] Bacillus licheniformis can promote the growth of normal physiological anaerobic bacteria in the intestine, adjust the imbalance of intestinal flora, restore intestinal function, and have special effects on intestinal bacterial infection. [0003] The existing preparation method of bacillus licheniformis has a single product, and the current technology still needs to be improved. Contents of the invention [0004] The purpose of the present invention is to provide a preparation method capable of preparing excellent bacillus licheniformis and a large number of other beneficial metabolites. [0005] To achieve the above object, the technical solution of the present invention is: provide a kind of preparation method of bacillus licheniformis, it is characterized in that, comprises the steps: [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/52C12P7/40C12P7/64C12N9/56C12N9/28C12R1/10
Inventor 廖庄旺
Owner XIAMEN LIUWEI BIO TECH
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