Stachys sieboldii stem tip detoxification and rapid propagation technology

Abstract

The invention relates to a Stachys sieboldii stem tip detoxification and rapid propagation technology, which comprises the steps of: 1) initiation culture of Stachys sieboldii rhizomes; 2) multiplication culture of Stachys sieboldii adventitious buds; 3) stem tip stripping and inoculation; 4) culture of Stachys sieboldii stem tip calluses; 5) differentiation of callus adventitious buds; 6) identification of virus-free seedlings; 7) rooting and transplanting of the virus-free seedlings; and 8) field planting. By applying the Stachys sieboldii stem tip detoxification technology, the Stachys sieboldii seedling adventitious bud incidence rate, the detoxification rate and the survival rate are all high, so that the technology can be used for promotion and planting to expand the per mu yield.

Description

technical field [0001] The invention relates to a virus-free propagation technology of caddis silkworm, in particular to a virus-free and rapid propagation technology of caddis silkworm stem tips. Background technique [0002] Grass caddis (Stachys sieboldii ) is one of the traditional vegetables in my country and has broad market development prospects. The stachyose (80g / kgDM) contained in its fleshy rhizome is a functional oligosaccharide that can significantly promote the proliferation of bifidobacteria. It is known as "Super strong bifidity factor". Traditionally, the accumulation and transmission of viruses through vegetative reproduction in rhizomes has seriously affected production. The production of seedlings under in vitro induced conditions can not only solve the problems of seed degradation and disease spread, but also save the land used for seed propagation. The continuous understanding of the medical, medicinal and health care values ​​of caddis silkworms ha...

AI Technical Summary

Problems solved by technology

The continuous understanding of the medical, medicinal and health care values ​​of caddis silkworms has favorably promoted the development of caddis silkworm industrialization and accelerated the development of caddis silkworm planting industry, especially in Hebei, Henan, Shaanxi and other provinces. The planting area increases sharply every year. The traditional cultivation measures of caddis worm and a single farm variety have been difficult to meet the production needs, and the occurrence of viral diseases is very common. The research on its cultivation technology and the planting application of virus-free plants are urgently needed in production. Establish an efficient regeneration system to realize industrial seedling supply, and solve the problems of low seedling emergence rate (30%~50%) and long seedling period of grass caddis in production

[0003] In the searched literature "Induction of Adventitious Buds and Establishment of Efficient Proliferation System of Cadidadidae" (Zhang Guoyu et al., "Journal of Northwest Agriculture and Forestry University"), the explants were studied using the stem tip, leaf and stem tissue of Caddisiac. The effect of different hormone ratios on the induction and proliferation of adventitious buds, the regeneration rate of larger adventitious buds directly induced by shoot tips is lower