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Liver organoid, uses thereof and culture method for obtaining them

An organoid, liver technology, applied in the culture process, tissue culture, cell culture active agent, etc.

Active Publication Date: 2013-08-07
KONINK NEDERLANDSE AKADE VAN WETENSCHAPPEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, liver cultures derived from hepatocytes or through differentiation of embryonic stem (ES) or induced pluripotent stem cells cannot expand and self-renew for longer periods of time

Method used

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  • Liver organoid, uses thereof and culture method for obtaining them
  • Liver organoid, uses thereof and culture method for obtaining them
  • Liver organoid, uses thereof and culture method for obtaining them

Examples

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Embodiment Construction

[0014] In a first aspect, the present invention provides a method of obtaining and / or culturing liver fragments or liver organoids, wherein the method comprises:

[0015] Epithelial stem cells and / or isolated tissue fragments containing said epithelial stem cells are cultured in contact with an extracellular matrix in the presence of a medium comprising minimal medium for animal or human cells supplemented with : epidermal growth factor and FGF as mitogenic growth factors capable of binding to FGFR2 or FGFR4 and preferably FGF10, nicotinamide, and preferably a Wnt agonist, preferably R-spondin1, R-spondin2, R-spondin3 or R-spondin4 and / or Wnt-3a. Preferably, HGF is also added.

[0016] For example, in one embodiment, the invention provides a method of obtaining and / or culturing a liver fragment or an organoid of the liver, wherein the method comprises:

[0017] Epithelial stem cells and / or isolated tissue fragments containing said epithelial stem cells are cultured in contac...

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Abstract

The invention relates to a liver organoid, uses thereof and a method for obtaining them.

Description

field of invention [0001] The present invention relates to liver organoids, their use and methods of obtaining them. Background of the invention [0002] The basic structural unit of the liver is the hepatic lobules. Each lobe consists of a hepatocyte plate lined with sinusoidal capillaries that line radially towards the central efferent vein. The hepatic lobules are roughly hexagonal, with each of the six corners divided by the presence of the portal triad (portal vein, bile duct, and hepatic artery). Although hepatocytes are the predominant parenchymal cell type of the liver, they are associated with cholangiocytes (biliary epithelial cells), endothelial cells, sinusoidal endothelial cells, Kupffer cells, natural killer cells, and Hepatic stellate cells work together. This complex structure is key to liver function. [0003] The existence of liver stem cells is still controversial. On the one hand, when a certain injury occurs, tissue maintenance and liver regeneratio...

Claims

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Application Information

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IPC IPC(8): C12N5/074
CPCC12N2501/155C12N2501/115C12N5/0672C12N2501/11C12N2500/38C12N2501/12C12N2533/90C12N2501/415A61K35/407C12N5/0671C12N2501/119A61P1/16C12N5/00C12N5/0662C12N5/067
Inventor 梅里特克赛尔·胡克奥尔特加约翰尼斯·卡洛鲁斯·克莱威尔斯
Owner KONINK NEDERLANDSE AKADE VAN WETENSCHAPPEN
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