Method for extracting purified oridonin from rabdosia rubescens

Abstract

The invention discloses a method for preparing oridonin from rabdosia rubescens. The method comprises the following steps of: (1), crushing rabdosia rubescens material, adding 60%-90% methanol solution for ultrasonically extracting for two to three times with each time being 20-50 minutes, combining and filtering, adding an ultra-filtration membrane for ultra-filtration, concentrating permeate in vacuum under reduced pressure to obtain a concentrated solution; (2), adding the concentrated solution to macroporous resin for adsorbing and evaporating moisture and packing, using a mixed solution of dichloromethane and acetone for eluting, collecting fraction components, and concentrating under reduced pressure; and extracting the concentrated solution by an ethyl acetate solution and collecting the extracting solution; (3), and purifying the concentrated extracting solution by adopting high-speed counter-current chromatography, carrying out online monitoring by an ultraviolet detector, collecting target components according to a figure, recycling a reagent and drying under reduced pressure to obtain the oridonin. The method for preparing the oridonin is simple to operate, high in product yield, high in purity, capable of recycling the reagent and low in production cost.

Description

technical field [0001] The invention belongs to the field of natural medicine chemistry, and in particular relates to a method for extracting and purifying oridonin from Rubescens sativa by high-speed countercurrent chromatography. Background technique [0002] Oridonin A is also called Oridonin, Yanming Caoning, tetracyclic diterpenoids, molecular formula C20H28O6, molecular weight 364.43, melting point 248°C-250°C, slightly soluble in water, soluble in methanol, ethyl acetate, acetone wait. [0003] The role of oridonin in cancer treatment has attracted people's attention, and it has been found that it has inhibitory effects on various cell lines such as lung cancer, prostate cancer, and breast cancer. [0004] Donglingcao is a perennial herb or subshrub of the Lamiaceae Fragrant Tea genus. Donglingcao has the effects of clearing heat and detoxifying, reducing inflammation and relieving pain, invigorating the stomach and activating blood circulation. [0005] Currently,...

AI Technical Summary

Problems solved by technology

These methods are all separated by silica gel column, the product loss is serious, the operation is cumbersome, and it is not suitable for industrialization