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Sample holding device

A technology for holding and sample, applied in the direction of biochemical cleaning device, enzymology/microbiology device, laboratory container, etc.

Inactive Publication Date: 2013-07-24
安泰凯生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these devices are specialized and require additional equipment

Method used

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Examples

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Embodiment Construction

[0027] Figure 1-5 A view of a sample holding device 100 according to an embodiment of the present invention is shown. The device 100 has a box-like shape comprising an upper portion 102 and a bottom 104 . One or more walls connect the upper and lower sections. Sections in the wall can have cutouts, as shown, or the wall can be continuous. The bottom 104 can include stepped edges arranged to match the edges of the upper portion. In this way, devices can be stacked above or below identical devices. The bottom 104 can have the imprint of a standard SBS microtiter plate.

[0028] Device 100 can be constructed from any material compatible with the desired testing procedure, eg, a transparent non-fluorescent material. In some embodiments, device 100 is constructed from a transparent material, such as a polymer (eg, polystyrene, polycarbonate, polypropylene) or glass. Device 100 can be constructed from multiple substructures, or fabricated on a continuous sheet of material suc...

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Abstract

A sample holding device having a plurality of sample wells. A gutter surrounds the sample wells and is in fluidic communication with an overflow sample well. An upper surface of the device is sealed with a sealing film that fluidically isolates each sample well from each other and from the gutter and overflow well. The device is used to perform a most probable number (MPN) procedure.

Description

technical field [0001] This application relates to sample holding devices. Background technique [0002] The most probable number (MPN) is a method of estimating the population density of viable microorganisms in a test sample. It is based on the application of probability theory to the number of observed positive growth to estimate the sample inoculum in a certain number of multi-tube media tubes from serial dilutions of cultured samples. A positive growth response after incubation can be indicated by observations such as gas production in fermentation tubes, visible turbidity in liquid culture tubes, a change in color of the liquid, or a fluorescent response when viewed under ultraviolet light, depending on the The type of media used. [0003] Typically, MPN testing is performed using several different sample holding containers of varying volumes. For example, when performing a 15-tube serial dilution method on MPN, each of the fifteen tubes needs to be filled with samp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/06
CPCB01L3/00C12Q1/06B01L3/5085B01L2200/025C12M23/12
Inventor 吴秋欣小欧内斯特·J·奥利佛拉斯黄永青
Owner 安泰凯生物技术有限公司
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