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External-circulation airlift type reactor adopting membrane tube aeration

An air-lift reactor and external circulation technology, which is applied in specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, biochemical instruments, etc., can solve the problem of unfavorable aerobic microbial fermentation, volumetric mass transfer coefficient low, limiting bacterial growth, etc., to achieve the effect of convenient disassembly and maintenance, small bubble diameter, and improved mass transfer performance

Inactive Publication Date: 2013-07-24
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide an external circulation airlift reactor using membrane tube aeration to solve the low volume mass transfer coefficient obtained by using nozzle type and orifice plate gas distribution in the existing external circulation airlift reactor , not conducive to aerobic microbial fermentation, limiting bacterial growth and metabolism

Method used

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  • External-circulation airlift type reactor adopting membrane tube aeration
  • External-circulation airlift type reactor adopting membrane tube aeration

Examples

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Effect test

Embodiment 1

[0020] figure 1 It is a structural schematic diagram of the present invention, figure 2 yes figure 1 The top view of A-A direction and B-B direction in the center, combined with figure 1 , figure 2 As shown, the external circulation airlift reactor using membrane tube aeration includes the external circulation airlift reactor 1, and the ascending zone and descending zone of the external circulating airlift reactor 1 are respectively provided with a plurality of fan-shaped plate fins Plate-fin heat exchanger 1 2 and plate-fin heat exchanger 2 10 of heat exchanger fins, fan-shaped plate-fin heat exchangers are used to exchange heat, figure 1 Shown is an external circulation airlift reactor with two rising zones. The upper end of the plate-fin heat exchanger-2 has steam inlet and cooling water outlet flange-3, and the lower end has steam condensate outlet and cooling water inlet flange-6. Among them, the steam inlet and cooling water outlet flange-3 pass through the ring T...

Embodiment 2

[0024] This example is used to illustrate the use of 10 m 3 The method for fermenting and producing L-lysine in a reactor using a membrane tube aeration method.

[0025] First, the culture medium is sterilized, and the stirring motor is turned on at a speed of 100 r / min, so that the fermentation broth is evenly heated. When the temperature rises above 95 degrees, the stirring can be stopped. Carry out the sterilization of the air filter and the air pipeline again, and slightly open the filter and the drain valve to ensure the sterilization of the air pipeline. Finally, the outlet, sampling valve, steam valve and outlet valve are slightly opened to ensure that the pipeline is sterilized.

[0026] Fermentation culture and results: Escherichia coli producing L-lysine (purchased from CGMCC, strain preservation number CGMCC1.0281) was inserted into a 5 m 3 10 m of fermentation medium 3 In a stirred reactor with membrane tube aeration (1L fermentation medium contains: glucos...

Embodiment 3

[0029] This example is used to illustrate the use of 50 m 3 The reactor adopts the method for fermenting and producing citric acid in a membrane tube aeration mode.

[0030] First, the culture medium is sterilized, and the stirring motor is turned on at a speed of 100 r / min, so that the fermentation broth is evenly heated. When the temperature rises above 95 degrees, the stirring can be stopped. Carry out the sterilization of the air filter and the air pipeline again, and slightly open the filter and the drain valve to ensure the sterilization of the air pipeline. Finally, the outlet, sampling valve, steam valve and outlet valve are slightly opened to ensure that the pipeline is sterilized.

[0031] Fermentation culture and results: According to the inoculation amount of 10%, citrate-producing Aspergillus niger (purchased from CGMCC, the preservation number of Aspergillus niger is CGMCC5343) was inserted into a 35m 3 50m of fermentation medium 3 In an aerated reactor using...

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Abstract

The invention relates to an external-circulation airlift type reactor adopting membrane tube aeration. The external-circulation airlift type reactor comprises an external-circulation airlift type reactor body, wherein a gas distributor is arranged on the bottom of the external-circulation airlift type reactor body; and a membrane tube with a nanoscale pore diameter is connected on the gas distributor. According to the external-circulation airlift type reactor adopting the membrane tube aeration, the membrane tube is applied to the external-circulation airlift type reactor, and the nanoscale pore diameter of the membrane tube is adopted, so air bubbles escaping from the membrane tube are small in diameter and large in quantity, and therefore, the problem that the air bubbles of a spray nozzle type distributor and a plate pore type distributor are larger is overcome; moreover, the mass transfer performance is greatly improved for facilitating the control of dissolved oxygen in fermentation liquor.

Description

technical field [0001] The invention belongs to the field of external circulation airlift reactors, and relates to an external circulation airlift reactor adopting membrane tube aeration. Background technique [0002] Airlift reactors are mainly divided into two categories, namely, internal circulation airlift reactors and external circulation airlift reactors. The two reactors are mainly distinguished by different fluid circulation paths. When aeration is performed in the upflow zone, the difference in apparent fluid density due to the difference in air holdup in the upflow and downflow zones will eventually cause the fluid to circulate outside the reactor. The external circulation airlift reactor can be regarded as composed of two gas-liquid bubbling beds and the connecting part between the top and the bottom of the two. [0003] The solubility of oxygen in water is low. Under normal temperature and pressure, the solubility of oxygen in pure water is 0.2mmol / L, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/04C12M1/02
Inventor 韦策李干禄吴兵李晖陈可泉欧阳平凯
Owner NANJING UNIV OF TECH
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