Cytomechanics device capable of synchronously realizing suction loading and fluorescence observation

Abstract

The invention discloses a cytomechanics device capable of synchronously realizing suction loading and fluorescence observation. The cytomechanics device comprises a microtubule suction module, an inverted fluorescence phase contrast microscope, a signal acquisition module and a control module, wherein the microtubule suction module is used for capturing, sucking and micro-controlling cells in a sample cell; the inverted fluorescence phase contrast microscope is used for fluorescently exciting the cells; the signal acquisition module is used for acquiring weak fluorescence signals; and the control module is used for synchronously triggering microtubule suction and fluorescence acquisition and conducting data analysis and treatment. The cytomechanics device breaks through the limit of time-space separation of the existing mechanical loading and optical detection method in cell-molecular biomechanics studies, and constructs a molecular-cell dynamics real-time in-situ observation system coupled with microtubule suction mechanical loading and fluorescence observation, thereby improving the platform for studying living cell and molecular mechanical behaviors, and contributing to deep understanding of the mechanics-chemistry and mechanics-biologics coupling rules from the molecular-cell level.

Description

technical field [0001] The invention relates to a cell mechanics device combining microtube sucking mechanics loading and microfluorescence observation. Background technique [0002] The human body is always in a mechanical environment, and its biological processes are regulated by different mechanical environments, showing basic characteristics such as multiple factors, nonlinearity, and interaction. It is necessary to quantitatively understand its coupling laws at the micro level. Cells are not only in a complex biochemical environment, but also in different biomechanical environments; cell mechanics can clarify how cells sense, modify, and respond to the physical characteristics of the cell environment; cells communicate through chemical and physical signals Realize information exchange, and thus participate in a series of biological processes such as embryogenesis, wound healing, inflammatory response, tumor metastasis; the response of cells to mechanical stimuli is very...

AI Technical Summary

Problems solved by technology

Mechanical loading experiment technology: applying mechanical effects to cells or molecules is to simulate the physiological and mechanical environment of cells or molecules, and it is impossible to realize the dynamic behavior of living cells (migration, proliferation, differentiation, etc.) and the cytoskeleton in living cells under mechanical stimulation , real-time dynamic detection of ionic and molecular activities and changes, and because cell culture, probe labeling and mechanical loading cannot be performed in situ, it is difficult to quantify test results and realize time-space coupling

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