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Selective medium additive capable of color development of staphylococcus aureus

A culture medium additive, Staphylococcus technology, applied in the direction of microbe-based methods, microbe measurement/inspection, microbiology, etc., can solve the problems of short storage time, high operator proficiency requirements, cumbersome preparation of culture medium, etc., and achieve extended Effects of storage time, avoiding quality control issues, and simplifying the media preparation process

Inactive Publication Date: 2013-07-10
苏州市第五人民医院
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  • Abstract
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  • Claims
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Problems solved by technology

At present, blood plate, high-salt high-mannitol plate medium, and Baird-Parker medium are mainly used to screen Staphylococcus aureus by using the hemolytic ring generated by Staphylococcus aureus and egg yolk reaction or egg yolk reaction positive tellurite reaction, but These methods have the problems of some staphylococcus aureus strains with insignificant hemolytic ring and egg yolk reaction, some staphylococcus aureus strains do not grow well in high-salt medium, high requirements for operator proficiency, cumbersome preparation of medium, short storage time, etc. , due to the use of additives such as egg yolk, it cannot be applied to simple test methods such as reagent sheets or test paper

Method used

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specific Embodiment

[0014] Take 15g of sodium chloride, 20g of mannitol, 0.25g of tellurite, 2.5g of lithium chloride, 5g of glycine, 2.5g of potassium phosphate, and 10mg of colistin sulfate, dissolve them in 50-100ml of deionized water, filter and sterilize. Additives for use; take 1000ml of nutrient agar and dissolve it in 900-950ml of deionized water, sterilize at 121°C under high temperature and high pressure, then cool to 50-60°C, add the above-mentioned additives, and pour it on a sterile flat medium plate to make golden yellow grapes Cocci selective chromogenic plate medium.

[0015] Take 3 clinical isolates of Staphylococcus aureus and the standard strain of Staphylococcus aureus ATCC25923, as well as several clinical isolates of Staphylococcus scalp, Staphylococcus hominis, Staphylococcus epidermidis, Streptococcus viridans, Escherichia coli, Klebsiella, etc. after inoculation Cultivate in the above-mentioned 37°C medium for 24 hours, and observe the result.

[0016] The results showed...

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Abstract

The invention discloses a selective medium additive capable of color development of staphylococcus aureus. Every 1000ml of a medium consists of the following ingredients by weight: 10-15g of sodium chloride, 10-20g of mannitol, 0.1-0.5g of tellurite, 1-5g of lithium chloride, 1-10g of glycine, 1-5g of potassium phosphate and 5-50g of colistine sulfate. According to the additive, animal blood yolk does not need to be used, so that the storage time of the medium is prolonged, a quality control problem which is caused by misusing contaminated animal blood yolk and the like can be avoided, and a medium preparation process is simplified; and the additive can be applied to both a plate medium and simple detection reagents such as test papers; when the selective medium additive is in use, basic nutritional ingredients such as peptone and yeast extract need to be added and uniformly applied onto non-woven fabrics and the like.

Description

technical field [0001] The invention relates to a medium additive, in particular to a selective medium additive capable of developing color of Staphylococcus aureus. Background technique [0002] Staphylococcus aureus (Staphyloccocus aureus) is an important pathogenic bacteria of humans, belonging to the genus Staphylococcus (Staphylococcus), has another name of "meatophilus", is a representative of Gram-positive bacteria, can cause many serious infections and food poisoned. At present, blood plate, high-salt high-mannitol plate medium, and Baird-Parker medium are mainly used to screen Staphylococcus aureus by using the hemolytic ring generated by Staphylococcus aureus and egg yolk reaction or egg yolk reaction positive tellurite reaction, but These methods have the problems of some staphylococcus aureus strains with insignificant hemolytic ring and egg yolk reaction, some staphylococcus aureus strains do not grow well in high-salt medium, high requirements for operator pro...

Claims

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Application Information

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IPC IPC(8): C12Q1/14C12R1/445
Inventor 贾宇玲吴妹英郦芳华金京勋赵静沈永林吴兴福
Owner 苏州市第五人民医院
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