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Alpinia kwangsiensis compound, as well as preparation method and application thereof

A compound, the technology of alpinia, which is applied in the fields of pesticides and phytochemistry, can solve the problems of separation that have not been seen yet, achieve good poisonous activity, broad application prospects, and simple preparation methods

Inactive Publication Date: 2013-07-10
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, only domestic scholars such as Nazhi have conducted research on the volatile oil components of Alpinia kwangsiensis, and no 5,6-dehydrokawain (5,6-dehydrokava piperonine) has been isolated from Alpinia kwangsiensis. ) reports
5,6-dehydrokawain has a variety of pharmacological activities, such as anti-oxidation, tumor cytotoxicity, antibacterial, inhibition of platelet aggregation, etc., but there are no reports of it being used as a plant-derived pesticide to kill nematodes

Method used

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  • Alpinia kwangsiensis compound, as well as preparation method and application thereof
  • Alpinia kwangsiensis compound, as well as preparation method and application thereof
  • Alpinia kwangsiensis compound, as well as preparation method and application thereof

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Embodiment 1

[0025] The Alpinia longifolia compound (5,6-dehydrokawain) of the present invention has a chemical structural formula as shown in Formula I:

[0026]

[0027] Formula I.

[0028] Preparation of Alpinia longifolium compound 5,6-dehydrokawain of the present invention

[0029] (1) Collect the root of Alpinia longifolium, dry it in the shade, crush it, and pass it through a 40-mesh sieve as raw material;

[0030] (2) Extract the pulverized Alpinia longifolia roots with analytically pure methanol as an organic solvent. The extraction is cold soaked for 3 times, each time for 1 day, and the extract is concentrated under reduced pressure to obtain a paste extract;

[0031] (3) Dissolving the paste extract and extracting it with ethyl acetate as an organic solvent to obtain the extracted product;

[0032] (4) Fraction X is collected after the extraction product is subjected to normal pressure silica gel column chromatography, and fraction X is subjected to normal pressure silica ...

Embodiment 3

[0037] Embodiment 3 The poisonous activity of Alpinia longifolium compound of the present invention to root-knot nematode

[0038] Use medicinal liquid dipping method to carry out activity measurement, first various glassware and utensils are sterilized, take Alpinia galanga compound 10mg, with distilled water (containing 5% methyl alcohol), be mixed with medicament mother liquor, the nematode suspension 7ml that prepares ( 50 pieces), added to the petri dish, and the corresponding amount of mother liquor was added, so that the concentration of each treated drug solution was 200 μg / ml, 100 μg / ml, 50 μg / ml, and repeated four times, and then the prepared petri dish Put it in a dark incubator at 25°C and RH60% for cultivation, observe with a stereo microscope at 72 hours, determine the number of dead nematodes by acupuncture, calculate the nematode mortality rate and corrected mortality rate, and refer to the five levels of nematicidal activity Standards are graded. The specific...

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Abstract

The invention discloses an alpinia kwangsiensis compound, as well as a preparation method and application thereof. The invention provides a chemical structural formula of the compound separated from alpinia kwangsiensis, and the compound is prepared from the root part of the alpinia kwangsiensis, has nematicidal activity and can be widely applied to preparing nematicidal agents. The preparation method disclosed by the invention has the advantages of simplicity, low preparation cost and broad application prospects.

Description

technical field [0001] The invention relates to the technical fields of pesticides and phytochemistry, in particular to a compound prepared from the plant Alpinia longifolium and its preparation method and application. Background technique [0002] Plant pathogenic nematodes mainly parasitize on various host plants such as vegetables, food crops, economic crops, and fruit trees, seriously affecting their yield and quality, and also aggravating the occurrence of soil-borne fungal diseases such as fusarium wilt and root rot and some bacterial diseases . Chemical control is the most common way to control plant pathogenic nematodes. Currently, the commonly used nematicides mainly include methyl bromide, carbofuran, thiazophos, and abamectin, but some of them are expensive to use, highly toxic, and seriously pollute the environment . Therefore, the research and development of new high-efficiency biological nematicides is the development trend of plant pathogenic nematode contro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D309/38A01N43/16A01P5/00
Inventor 徐俊驹秦小萍卢红杨生超赵磊峰
Owner YUNNAN AGRICULTURAL UNIVERSITY
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