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Application of lamprey Lj-RGD2 in preparation of anti-angiogenesis antitumour drug

An anti-tumor drug and angiogenesis technology, applied in the direction of anti-tumor drugs, applications, drug combinations, etc.

Inactive Publication Date: 2013-06-26
LIAONING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Lj-RGD2 is an RGD motif protein found in the oral gland of Japanese lamprey. It consists of 69 amino acid residues and has two RGD motifs. There is no research and report on this protein at home and abroad. This invention is the first Find

Method used

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  • Application of lamprey Lj-RGD2 in preparation of anti-angiogenesis antitumour drug
  • Application of lamprey Lj-RGD2 in preparation of anti-angiogenesis antitumour drug
  • Application of lamprey Lj-RGD2 in preparation of anti-angiogenesis antitumour drug

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Experimental program
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Embodiment Construction

[0017] 1. The Lj-RGD2 gene was connected with the pET23b vector, and transformed into Escherichia coli to screen and identify positive transformants.

[0018] In order to produce a fusion protein with a histidine tag, pET-23b was selected as the vector for gene cloning. The specific operation of cloning is as follows:

[0019] (1) Recovery and sequencing of the target gene: the target gene was recovered using TaKaRa PCR Fragment Recovery Kit; the recovered DNA was sequenced, and this work was completed by Dalian Bao Biological Engineering Co., Ltd.

[0020] (2) Plasmid extraction: The plasmid extraction kit of Baobio was used.

[0021] (3) Ligation of the target gene DNA fragment and the vector pET23b: Since the designed primers have Nde I and Hind III restriction sites respectively, and these two restriction sites are also multiple cloning sites of pET23b, this makes the It is possible to connect the DNA fragment of the target gene with the vector pET23b.

[0022] (4) the c...

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Abstract

The invention provides an application of lamprey Lj-RGD2 in preparation of an anti-angiogenesis antitumour drug, belongs to the biotechnology field and relates to gene cloning and protein expression of RGD die body protein Lj-RGD2 in an oral cavity gland of lamprey in Japan, a strong-effect function of Lj-RGD2 for inhibiting angiogenesis by inhibiting a signal transduction pathway of integrin highly expressed on the surface of new vascular endothelial cells and an application of the lamprey Lj-RGD2 as an angiogenesis inhibitor in preparation of antitumour drugs.

Description

technical field [0001] "Application of lamprey Lj-RGD2 in anti-angiogenesis anti-tumor drugs" belongs to the field of biotechnology, involving the gene cloning and protein expression of RGD motif protein Lj-RGD2 in the oral gland of Japanese lamprey, and rLj- RGD2 potently inhibits angiogenesis function by inhibiting the signal transduction pathway of integrin highly expressed on the surface of neovascular endothelial cells and its application as an angiogenesis inhibitor in anti-tumor pharmaceuticals. Background technique [0002] Angiogenesis refers to the process of forming new blood vessels from pre-existing blood vessels or pre-endothelial cells under the induction of growth factors. The growth and development of primary solid tumors are highly dependent on angiogenesis. Avascular tumors rarely grow more than 2 to 3 mm 3 of. Once a tumor becomes vascularized, it grows rapidly. In preclinical models, anti-angiogenic drugs targeting tumor vasculature have been shown t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/46A61K38/17A61P35/00
Inventor 李庆伟王继红吕莉刘洋
Owner LIAONING NORMAL UNIVERSITY
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