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Lactobacillus strain and application thereof

A technology of lactobacillus and strains, applied in the direction of bacteria, biological sludge treatment, microorganisms, etc., can solve the problems of complex microbial deodorant process methods, complicated deodorant methods, and poor deodorizing effect, and achieve deodorizing operation Simple and easy to operate, good on-site deodorization effect, stable deodorization effect

Active Publication Date: 2014-11-19
杨凌未来中科环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The deodorization stability of mixed strains is generally higher than that of single strains, but the method of preparing deodorants from mixed strains is more complicated. Generally, single strains are fermented first, and then mixed in a certain proportion to form microbial deodorants.
Because the physiological and biochemical characteristics, respiratory metabolism types and functions of each single strain of mixed strains are quite different, each single strain must have its own cultivation method, so the process of preparing microbial deodorant from mixed strains is more complicated and the cost is higher
However, when a single strain is used for deodorization, it has the disadvantage of poor deodorization effect, and its application is limited.

Method used

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  • Lactobacillus strain and application thereof
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  • Lactobacillus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The lactobacillus CGMCC6495 of the present invention is applied to feces deodorization.

[0018] 1. Preparation of culture medium and inoculation solution

[0019] Solid activation medium: peptone 10g, beef extract 10g, yeast extract 5g, glucose 5g, sodium acetate 5g, diammonium citrate 2g, Tween 801ml, magnesium sulfate 0.58g, manganese sulfate 0.05g, dipotassium hydrogen phosphate 2g, agar 15g~20g, add water to 1000ml, pH6.2. Sterilize at 121℃ for 30min.

[0020] Liquid expanded fermentation medium: peptone 5g, beef extract 5g, yeast extract 5g, glucose 10g, sodium acetate 5g, diammonium citrate 2g, Tween 801ml, magnesium sulfate 0.58g, manganese sulfate 0.05g, dipotassium hydrogen phosphate 2g, Make up water to 1000ml, pH6.0. Sterilize at 121°C for 30min.

[0021] Put Lactobacillus CGMCC6495 into the activation medium for activation and culture for 48 hours to obtain the activated strains for use; inoculate the activated strains into the expanded fermentation mediu...

Embodiment 2

[0028]Lactobacillus CGMCC6495 is applied to the deodorization of feces. It is the same as Example 1 and will not be repeated. The difference lies in the treatment conditions after the bacteria solution is inoculated into the feces.

[0029] After the bacteria liquid was inoculated into the feces, the beaker was sealed with plastic wrap, and the control treatment was carried out at the same time. The temperature was 20°C, and the odor intensity, ammonia gas concentration, and hydrogen sulfide concentration were measured after 5 days. The measurement results are shown in Table 2 below.

[0030] Table 2 Deodorization effect of Lactobacillus CGMCC6495 strain (20°C)

[0031]

[0032] It can be seen from the above table that when the temperature is 20°C, after 5 days of adding Lactobacillus CGMCC6495 bacterial solution to the feces, the odor intensity is reduced by 22.0%, the ammonia concentration is reduced by 28.9%, and the hydrogen sulfide concentration is reduced by 20.7%. C...

Embodiment 3

[0034] Lactobacillus CGMCC6495 is used in feces deodorization, and the same as Example 1 will not be repeated, the difference lies in the inoculation amount. The inoculum volume (V / W) was 1%, 6.66%, and 10%, respectively, and the measurement results at the end of the treatment are shown in Table 3.

[0035] Table 3 Deodorization effect of Lactobacillus CGMCC6495 strain (30℃±5℃)

[0036]

[0037] It can be seen from the above table that after adding different inoculum amounts of Lactobacillus CGMCC No.6495 to the feces for 5 days, the deodorizing effect showed a trend of first increasing and then decreasing with the increase of inoculum amount. The odor effect is the best, the odor intensity is reduced by 36.1%, the ammonia concentration is reduced by 41.4%, and the hydrogen sulfide concentration is reduced by 43.3%.

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Abstract

The invention discloses a lactobacillus strain and application thereof. The lactobacillus CGMCC6495 is separated from pickled vegetable wastewater and stored in a common microorganism center of China Committee for Culture Collection of Microorganisms with the collection date of September 3, 2012 and the collection number of CGMCC No.6495. The strain can be applied to biological deodorization of excrement through the conventional activation culture and enlargement fermentation cultivation; the lactobacillus CGMCC 6495 can be used as a single strain for in-situ deodorization of the human excrement, so that the deodorization effect is stable and the temperature influence is small. Under the normal-temperature condition, the lactobacillus strain has a better deodorization effect in both a laboratory environment and a public toilet site environment.

Description

technical field [0001] The invention relates to a lactobacillus strain, in particular to a lactobacillus strain in the field of environmental biotechnology and an application thereof. Background technique [0002] The increasing number of urban public toilets and public toilets in scenic spots has become an important symbol of the management level and civilization of towns and scenic spots, but the odor pollution caused by it has become an important issue of environmental protection in cities and scenic spots. The stench of feces contains a variety of toxic components, among which ammonia, hydrogen sulfide, mercaptan, and methyl mercaptan are the main harmful gas components, which will cause serious harm to people's health after long-term inhalation. The deodorization level of public toilets is not only related to environmental sanitation, public health, air pollution, but also to the reputation and external image of towns and scenic spots. [0003] At present, there are ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/225
Inventor 闫志英刘晓风袁月祥廖银章李志东许力山
Owner 杨凌未来中科环保科技有限公司
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