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Terahertz spectrum detection method for aflatoxin

A technology of aflatoxin and detection method, which is applied in the field of physical detection of trace harmful substances, can solve the problems of complex instruments and equipment, difficult to achieve rapid on-site analysis, and cumbersome, and achieves the effect of simple detection process, shortened detection time, and risk avoidance.

Inactive Publication Date: 2015-04-22
HENAN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

The instruments and equipment are complex and heavy, making it difficult to achieve rapid on-site analysis

Method used

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  • Terahertz spectrum detection method for aflatoxin
  • Terahertz spectrum detection method for aflatoxin
  • Terahertz spectrum detection method for aflatoxin

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Embodiment Construction

[0020] The present invention will be described in detail below in conjunction with specific embodiments.

[0021] The specific embodiment of the terahertz spectrum detection method of aflatoxin of the present invention is as follows:

[0022] 1. Homogenized sample

[0023] Remove impurities from solid samples (such as peanuts, soybeans, wheat, etc.), soak them in water, and after the samples are soft, take out the samples and put them in a blender to stir for 1-2 minutes to make them homogeneous at high speed. For powdery samples, add water to the Erlenmeyer flask and vibrate on a shaker for 30 minutes for homogenization.

[0024] 2. Rough filtration and dilution

[0025] The homogeneous solution was filtered through fluted filter paper, 15ml of the filtrate was accurately pipetted and diluted with 30ml of water.

[0026] 3. Pass the sample through the column

[0027] Connect the immunoaffinity column under the glass syringe, and accurately pipette 15ml of the sample extra...

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Abstract

The invention discloses a terahertz spectrum detection method for aflatoxin. The terahertz spectrum detection method comprises the steps of: A1. homogenizing of a sample; A2. coarse filtration and dilution; A3. column chromatography on the sample; A4. cleaning of impurities; A5. elution; A6. preparation and preservation of a sample to be detected; A7. preparation of a sample for terahertz trace detection; A8. terahertz time-domain spectroscopy detection on the sample; A9. establishment of an aflatoxin terahertz spectrum library; and A10. recognition of aflatoxin types based on terahertz spectrum detection, comparison the terahertz spectrum of the sample obtained in A8 with the spectrum in the aflatoxin spectrum library, so as to obtain the aflatoxin type with highest correlation by adopting a template matching method, namely the type of the aflatoxin in the sample. By utilizing the terahertz spectrum detection method to detect the aflatoxin, the terahertz spectrum detection method has the prominent advantages of being high in detection speed, higher in intelligence compared with that of a common biochemical detection method, simple in the detection process, and simple and convenient to operate.

Description

technical field [0001] The invention relates to a new method for physical detection of trace harmful substances, in particular to a terahertz spectrum detection method for aflatoxin. Background technique [0002] Aflatoxin is an item that must be inspected by the state in food safety inspections. At present, biochemical detection methods are mostly used. This type of detection method also has defects in one way or another. For example, although the TLC method is simple, but the sensitivity is poor; although the HPLC method has high sensitivity, the sample processing is cumbersome, the operation is complicated, and the instrument is expensive. ELISA has poor reproducibility and short reagent life requires low temperature storage. During operation, most of these methods require the use of highly toxic mycotoxins as calibration standards, which poses a risk of causing huge contamination to operators. In the process of sample pretreatment, it is necessary to use a variety of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/25
Inventor 徐朝辉廉飞宇付麦霞翟焕趁张元白浩
Owner HENAN UNIVERSITY OF TECHNOLOGY
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