Method for preparing photosynthetic bacteria liquid

Abstract

The invention discloses a method for preparing rhodovulum sulfidophilus liquid. The method includes the following steps: (1) a cryopreserved tube containing the rhodovulum sulfidophilus liquid is dissolved and revived, the dissolved bacteria liquid comes to be revived bacteria liquid; (2) on an ultra-clean working platform, by utilization of the revived bacteria liquid, streaking is carried out on a flat plate solid medium, the streaked flat plate solid medium is placed in a constant temperature incubator with the temperature of 30 DEG C for being cultivated for 24-36 hours in a static mode, after single colonies are grown, an inoculating loop is utilized to pick the single colonies on the flat plate solid medium to streak on a slope culture medium, the streaked slope culture medium is placed in the incubator with the temperature of 30 DEG C for being cultivated for 24-36 hours in a static mode, and bacterial colonies are grown; and (3) after the bacterial colonies are grown, by utilization of a rhodovulum sulfidophilus liquid culture medium, the bacterial colonies on the slope culture medium are eluted to be bacterial colony liquid, the bacterial colony liquid is cultivated for the first time so as to obtain first-time culture bacterial liquid, and transfer culture is carried out on the first-time bacterial liquid to obtain transfer culture bacterial liquid. The method for preparing the rhodovulum sulfidophilus liquid has the advantages of being easy in preparation and culture of the culture media, high in the bacteria liquid concentration and good in quality.

Description

technical field [0001] The invention relates to a method for preparing a bacterial liquid, in particular to a method for preparing a photosynthetic bacterial liquid. Background technique [0002] Photosynthetic bacteria (photosynthetic bacteria, referred to as PSB), is the oldest prokaryote on the earth with a primitive photosynthetic system, is a general term for a type of bacteria that photosynthesize under anaerobic conditions and does not produce oxygen, which is related to green plants , algae and other photosynthetic organisms. Photosynthetic bacteria are widely distributed in swamps, ponds, lakes, rivers, ditches, oceans and soils, and are excellent aquatic environment improvers and feed additives. At present, the preparation methods of photosynthetic bacteria liquid are very cumbersome, and the composition of the medium is complicated. Most of them are cultivated in special medium, and they are cultivated under a certain pH value and light intensity. It is often ne...

AI Technical Summary

Problems solved by technology

At present, the preparation methods of photosynthetic bacteria liquid are very cumbersome, and the composition of the medium is complicated. Most of them are cultivated in special medium, and they are cultivated under a certain pH value and light intensity. It is often necessary to add one or some special substances to the culture medium, such as starter, papaya extract, mistletoe extract, etc., the composition is complex, the preparation is cumbersome, and most of the preparation process requires special light.

Images