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Rapid breeding method for malus micromalus tissue culture

A technology of tissue culture and Begonia, which is applied in the field of rapid plant propagation, can solve the problems of small reproduction coefficient, limited success of tissue culture to individual species, slow research progress, etc., and achieves the effect of superior tissue growth conditions and avoiding tissue browning

Inactive Publication Date: 2013-06-12
王茜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional Xifu crabapple propagation method mostly adopts division propagation method, the reproduction coefficient is small, the degree of industrialization is low, and it is far from meeting the market consumption demand
Although there are some research reports on tissue culture of Begonia Xifu in China, the success of tissue culture is limited to individual species, and the growth of protocorms is easy to brown, so the research progress is slow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Inoculate the young shoots of crabapple plants on the induction medium to induce initial protocorms the size of sesame seeds; the specific composition and content of the induction medium are: KN0 3 1200mg / L, NH 4 N0 3 1000mg / L, KH 2 P0 4 110mg / L, MgS0 4 230mg / L, CaCl 2 200mg / L, KI 0.83mg / L, MnS0 4 22.3mg / L, ZnS0 8.6mg / L, Na 2 -EDTA 37.3mg / L, FeS0 4 27.8mg / L, 20-30mg / L sucrose, 0.01-0.5mglL 6-BA, 0.01g / L activated carbon, 4g / L agarose, pH 5.0;

[0018] Two, the initial protocorm is transferred to the proliferation medium for proliferation culture, every four weeks, after the proliferated initial protocorm is cut, subculture is carried out, so that the protocorm proliferates rapidly, and the medium used for subculture is the same as described The proliferation medium is the same; the proliferation medium is a liquid-solid two-phase medium with improved MS as the basic medium and additional components, in which the upper layer is a liquid phase medium...

Embodiment 2

[0022] 1. Inoculate the young shoots of crabapple plants on the induction medium to induce initial protocorms the size of sesame seeds; the specific composition and content of the induction medium are: KN0 3 1300mg / L, NH 4 N0 3 1200mg / L, KH 2 P0 4 120mg / L, MgS0 4 250mg / L, CaCl 2 250mg / L, KI 0.83mg / L, MnS0 4 22.3mg / L, ZnS0 4 8.6mg / L, Na 2 -EDTA 37.3mg / L, FeS0 4 27.8mg / L, sucrose 25mg / L, 6-BA 0.2mg / L, activated carbon 0.08g / L, agarose 4.5g / L, pH 5.2;

[0023] Two, the initial protocorm is transferred to the proliferation medium for proliferation culture, every four weeks, after the proliferated initial protocorm is cut, subculture is carried out, so that the protocorm proliferates rapidly, and the medium used for subculture is the same as described The proliferation medium is the same; the proliferation medium is a liquid-solid two-phase medium with improved MS as the basic medium and additional components, in which the upper layer is a liquid phase medium and t...

Embodiment 3

[0025] 1. Inoculate the young shoots of crabapple plants on the induction medium to induce initial protocorms the size of sesame seeds; the specific composition and content of the induction medium are: KN0 3 1500mg / L, NH 4 N0 3 1350mg / L, KH 2 P0 4 140mg / L, MgS0 4 300mg / L, CaCl 2 280mg / L, KI 0.83mg / L, MnS0 4 22.3mg / L, ZnS0 4 8.6mg / L, Na 2 -EDTA 37.3mg / L, FeS0 4 27.8mg / L, 30mg / L sucrose, 0.5mg / L 6-BA, 0.1g / L activated carbon, 5g / L agarose, pH 5.4;

[0026] Two, the initial protocorm is transferred to the proliferation medium for proliferation culture, every four weeks, after the proliferated initial protocorm is cut, subculture is carried out, so that the protocorm proliferates rapidly, and the medium used for subculture is the same as described The proliferation medium is the same; the proliferation medium is a liquid-solid two-phase medium with improved MS as the basic medium and additional components, in which the upper layer is a liquid phase medium and the ...

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Abstract

The invention discloses a rapid breeding method for malus micromalus tissue culture, relates to rapid plant breeding methods, and belongs to the technical field of plant tissue culture. The rapid breeding method is characterized by comprising the following steps of: inoculating the bud of the malus micromalus on an induction medium so as to induce an initial protocorm which is as small as a sesame seed; transplanting the initial protocorm to an enrichment medium to carry out enrichment cultivation; and cutting the enriched e initial protocorm every four weeks to carry out subculture so as to rapidly enrich the protocorm. The rapid breeding method has the beneficial effects that the selected culture medium has both the advantages of a liquid culture medium and a solid culture medium, so that the advantage that the protocorm or the similar protocorm grows healthy and strongly in the solid culture medium is maintained, the advantage that the liquid culture medium is rapidly enriched is brought into play; and moreover as the protocorm or the similar protocorm is planted in the solid culture medium, the phenomenon of tissue browning caused by liquid culture medium shake or even rolling friction is avoided, and the tissue growing condition is more advantageous.

Description

technical field [0001] The invention relates to a rapid propagation method for plants, which belongs to the technical field of plant tissue culture, in particular to a rapid propagation method for crabapple tissue culture. Background technique [0002] Xifu crabapple (Malus spectabilis), also known as small fruit crabapple, is said to be named after it grew in Xifu during the Jin Dynasty. Small tree with oblong leaves and umbel-shaped racemes. When the flowers are not in bloom, the flower buds are bright red, like rouge, and gradually turn pink after blooming. The flower shape is relatively large, with 4 to 7 flowers in clusters upward, and the pear fruit is spherical. red. Now Liaoning, Hebei, Shanxi, Shandong, Shaanxi, Gansu and other northern regions are mostly cultivated. Begonias not only have gorgeous flowers and colors, but also have exquisite and impressive fruits. The fruit matures in autumn, and the red and yellow are correspondingly unique. Begonia has strong...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王茜
Owner 王茜
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