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Novel method for detecting citric acid fentanyl anaesthetic

A technology for fentanyl citrate and anesthetics, which is applied in sensitive and online detection of fentanyl citrate anesthetics, a new rapid field, can solve the problems of experimental repeatability, poor selectivity, popularization, application limitations, etc. Achieve the effect of fast measurement speed, simple measurement method and convenient portability

Inactive Publication Date: 2013-06-05
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the special reagents required for the detection of plasma fentanyl by immunoassay are time-sensitive, and the repeatability and selectivity of the experiment are poor. Most of the test results need to be verified, which limits the promotion and application of this method.

Method used

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  • Novel method for detecting citric acid fentanyl anaesthetic
  • Novel method for detecting citric acid fentanyl anaesthetic
  • Novel method for detecting citric acid fentanyl anaesthetic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Use a syringe to take 1-10 μL, 0.5-50 ppm fentanyl citrate anesthetic sample solution. In the positive ion mode, the ion mobility spectrometer is used as the detection instrument, and the carrier gas carries the anesthetic sample into the ionization region of the ion mobility spectrometer; the sample is ionized into positive and negative ions in the ionization region, and enters the ionization region by a uniform electric field through the periodically opened ion gate. The formed drift region is separated and detected in the drift region. Different compounds have different mobility constants, move at different speeds in the drift region, and take different times to reach the detector. By measuring the time of ion arrival at the detector and the peak intensity, the compound can be identified and its amount determined based on the signal intensity of the peak.

[0033] figure 1 The ion mobility spectrogram of the detection background of the positive ion mode ion mobilit...

Embodiment 2

[0037] In the positive ion mode, the ion mobility spectrometer is used as the detection instrument. The sample gas is carried into the ionization region of the ion mobility spectrometer by the carrier gas. The flow rate of the carrier gas is 400mL / min; The opened ion gate enters the drift region formed by a uniform electric field, and is separated and detected in the drift region. During the experiment, the transfer tube temperature was 100°C, the injector temperature was 180°C, and the carrier gas (air) and drift gas (air) flow rates were 400mL / min and 600mL / min, respectively.

[0038] Take 1μL of 0.5ppm, 1ppm, and 10ppm respectively with a 1μL microsampler, namely 0.5ng, 1ng, and 10ng; use a 5μL microsampler to take 2μL, 4μL, and 6μL of 10ppm fentanyl citrate anesthetic samples respectively , 8μL, that is, 20ng, 40ng, 60ng, 80ng; the sample piece was evaporated by the heater at 20-50°C to remove the solvent, and then entered the ion mobility spectrometer for analysis. 0.5ng...

Embodiment 3

[0041] image 3 The fitted relational formula can be entered into the editing formula in the software toolbar or automatically transferred into the system, and the instrument can automatically alarm and display recognition. One is that the alarm shows the presence or absence of fentanyl citrate anesthetic samples, which is realized through the alarm indicator light on the terminal interface; the other is to calculate the fentanyl citrate anesthetic samples directly in the toolbar according to the fitted formula, Displayed in the toolbar for semi-quantitative analysis.

[0042] Figure 4 Alarm software interface. As shown in the figure: 50ppm solution, injection volume 1μL (ie 50ng). The software alarm shows 53ng, and the relative deviation of this test is less than 10%.

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Abstract

The invention discloses a novel method which is capable of detecting citric acid fentanyl anaesthetic quickly and sensitively. The method takes ionic migration spectrometry technology as basic detection technology, a positive ion mode is adopted, and a qualitative and semi-quantitative analytical method for the citric acid fentanyl anaesthetic detected by the ionic migration spectrometry is created. Coupons containing different citric acid fentanyl anaesthetic samples are sent to be detected in a thermal desorption sample injector of an ionic migration spectrometry detector after solvent of the coupons is dried, detection signals are obtained, and a qualitative and semi-quantitative analysis is carried out. A sample measurement detection limit of the citric acid fentanyl anaesthetic can reach 50 parts per billion (ppb), a quantitative analysis range is between 1ng and 60 ng, a created standard curvilinear equation is that y is equal to 1.3451 plus 0.4044 x, and a correlation coefficient r is larger than 0.99. The detection method is simple, convenient to achieve, quick, high-efficient and good in reliability, and can be used for quick detection of the citric acid fentanyl anaesthetic in clinical diagnosis of a patient or on a crime scene.

Description

technical field [0001] The invention relates to an on-line detection method for fentanyl citrate anesthetics, in particular to a new fast, sensitive and on-line detection method for fentanyl citrate anesthetics. The method is based on the ion mobility spectrometry technology, using the ion mobility spectrometer fast detector as the analysis method, and the detection limit of the fentanyl citrate anesthetic can reach 50ppb. The volume of the instrument is small, the analysis time is short, and qualitative and semi-quantitative analysis can be performed. The measuring method is simple, fast, efficient and reliable. The method has fast analysis speed, simple test operation, no complicated sample pretreatment experiment, and no need for special professional background personnel to operate. Background technique [0002] Fentanyl citrate, the chemical name is N-[1-(2-phenylethyl)4-piperidinyl]-N-phenyl-propionamide citrate, the molecular formula is C 22 h 28 N 20 ·C 6 h 8 o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N27/622
Inventor 李海洋王新李林李京华梁茜茜渠团帅
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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