Tissue culture breeding method for hempleaf groundsel herb
A technology of tissue culture and soul-returning grass, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as shortening the cultivation period, and achieve the effects of shortening the growth period, short growth period and less material.
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[0019] The present invention will be further described below in conjunction with specific examples.
[0020] The tissue culture breeding method of Resurrection Grass comprises the following steps:
[0021] (1) Medium preparation: select MS medium as the basic medium, 6-benzylaminopurine (6-BA), naphthaleneacetic acid (NAA), gibberellin, zeatin (ZT) and 3-indoleacetic acid (IAA ) as a regulatory hormone to prepare three culture media:
[0022] Primary differentiation medium A: MS+6-BA0.4mg / L+ gibberellin 3mg / L,
[0023] Secondary differentiation medium B: MS+6-BA0.5mg / L+ ZT0.5mg / L,
[0024] Rooting medium C: MS +NAA0.4mg / L+IAA0.5mg / L,
[0025] Add 5% sucrose and 1% agar to medium A and B, add 4% sucrose and 0.5% agar to medium C, adjust the pH value to 6.0 with 1mol / L NaOH and 1mol / L HCl, and put the medium A, B and C are sterilized in a high-pressure steam sterilizer at 0.1MPa and 120°C for 20min.
[0026] (2) Disinfection of explants: choose freshly germinated young leav...
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