Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Trichodermalongibrachiatum GF-10 and application thereof

A technology of Trichoderma longibrachiae, which is applied in the direction of application, fungicides, fungi, etc., can solve the problems of no relevant reports on biological control, and achieve the effects of easy cultivation and preservation, reasonable technical route, and strong tolerance

Inactive Publication Date: 2013-05-29
BIJIE COMPANY OF GUIZHOU TOBACCO +1
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And there is no relevant report on the biological control of the above two diseases by Trichoderma longibrachiata

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] The separation steps of the Trichoderma longibrachiata strain GF-10 that has inhibitory effect on tobacco black shank bacterium and Alternaria Alternaria:

[0032] (1) Tested strain: black shank of tobacco - Phytophthora nicotiana ( Phytophthora nicotianae Gzufp-25), Tobacco Alternaria Alternaria ( Aiternaria alternate Gzufc-18) was isolated in Bijie City and preserved in the Institute of Fungal Resources, College of Life Sciences, Guizhou University.

[0033](2) Isolation and purification of antagonistic bacteria: Accurately weigh 10.0g of tobacco plant root soil sample collected from Qianxi County, Bijie City, Guizhou Province, and use aseptic technique to add the soil sample to 90mL sterile water with glass Shake it for 10 minutes to make the soil sample evenly disperse into a dilution, then pipette 1mL of the soil dilution into 9mL of sterile water and mix well to form a soil suspension. Use the mixed bacteria method to inoculate, draw 1mL of the suspension int...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses trichodermalongibrachiatum GF-10 and an application thereof. The morphological characteristics are as follows: the trichodermalongibrachiatum GF-10 grows rapidly on a PDA (Potato Dextrose Agar) and the back face grows yellow green from colorless; conidiums can be generated after the trichodermalongibrachiatum GF-10 is cultured at 28 DEG C for 72 hours; stem secondary branches of the conidiums are fewer and stretch out in a right angle generally; bottle stems directly stretch out from the secondary branches; solitary and dichotomy exist and whorls are very few; the bottle stems are in a calabash or flask type; the length is 5.3-11.6 microns and the width is 2.0-3.2 microns; the final bottle stem is conical; spores are grey green or green, and are oval or ellipse-shaped; and the length is 3.4-6.6 microns and the width is 2.3-3.5 microns. The trichodermalongibrachiatum GF-10 has strong tolerance on a bad environment, resists mutation, is easily cultured and stored, and has good effects on inhibiting phytophthora nicotiana and alternaria alternate.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Trichoderma longibrachiata strain, and also relates to the use of the Trichoderma longibranchium strain in inhibiting tobacco black shank and tobacco red spot fungus. [0002] Background technique [0003] Tobacco black shank is the most important rhizome disease in tobacco leaf production, while tobacco red spot is an important leaf disease in tobacco leaf production. The control of both is particularly important for tobacco leaf production. [0004] At present, there are many control methods for tobacco black shank and tobacco red spot, including cultivating disease-resistant varieties, chemical control, crop rotation and biological control. Planting disease-resistant varieties is the most economical and effective way to prevent and control the two diseases. However, when using disease-resistant varieties to prevent and control the two diseases, not only the resistance level and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 葛永怡李祝张传平庄培林黄化刚黄青明申燕孙希文刘阳
Owner BIJIE COMPANY OF GUIZHOU TOBACCO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com