Preparation method of ice-induced microstructure soft tissue

Abstract

The invention discloses a preparation method of an ice-induced microstructure soft tissue, and belongs to the field of biological tissue engineering. The method comprises the steps of firstly, designing a model with a soft tissue channel mesh microstructure; preparing a resin die with the channel microstructure by a material increase manufacturing technology; pouring pure water into the resin die and stripping after freezing, so as to obtain an ice-induced die with the channel microstructure; finally pouring PVA (polyvinyl alcohol) hydrogel with tissue living cells, growth factors and the like configured in advance into an ice-induced die system at low temperature; putting in an environment at 5-10 DEG C after stably molding until the ice-induced channel is slowly and completely molten, and finally obtaining the soft tissue with the channel microstructure. The situation that the channel structure does not deform because of external effects before being molded is ensured by the method; pollution to an original body tissue caused by high-temperature treatment is avoided; and the preparation process is simple and easy to operate, low in preparation method and wide in applicability.

Description

Technical field: [0001] The invention relates to a preparation method of artificial organ soft tissue used for internal organ replacement. The method is a preparation method of tissue engineering three-dimensional soft tissue and belongs to the technical field of bioengineering. Background technique: [0002] There are more than tens of millions of patients suffering from tissue defects or organ failure in the world each year. In the United States alone, approximately 8 million such patients are treated with surgery each year. However, the most effective way to solve this problem is to artificially replace the defective organ. There are two main ways to artificially substitute organisms: one is the use of autologous or allogeneic organ transplantation. However, the lack of donors and rejection have always restricted tissue and organ transplantation; the other is the use of artificial organs. Traditional artificial organs are usually made of metal, ceramic or polymer materials. ...

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Problems solved by technology

However, this method still has the following problems and deficiencies: First, it is necessary to design and manufacture the negative structure of the scaffold, which prolongs the production time of the scaffold and increases the cost; secondly, the scaffold needs to be treated at high temperature, which invisibly increases the probability of contamination of the biological scaffold ; Finally, the method cannot be used to fabricate soft scaffolds for artificial organs

However, this method still has the following problems and deficiencies: the internal three-dimensional channel structure of the living tissue prepared by this method cannot be completely satisfied, because it cannot bear the load before the internal channel structure is finalized, so the actual preparation There is still a big difference between the channel structure in the process and the pre-designed three-dimensional channel structure; in addition, there is still some difficulty in direct forming of the channel, and the internal channel of the soft support has certain requirements for the channel size

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