New method for preparing frozen semen of giant panda

Abstract

The invention discloses a new method for preparing frozen semen of a giant panda. Liquid nitrogen (3) is poured into a refrigerator body (5) after upper and lower layers of freezing brackets are put inside the refrigerator body (5); fine tubes (2) containing the frozen semen of grown giant panda are orderly arranged on the upper and lower layers of freezing brackets when the top end face of the upper layer of freezing bracket (1) achieves -100 to -70 DEG C; the fine tubes (2) are moved into the liquid nitrogen (3) inside the refrigerator body (5) after the fine tubes (2) are arranged for 30-90 seconds; and then the fine tubes (2) are arranged inside a liquid nitrogen tank to store for a long period of time after balance. The primary freezing temperature of the giant panda semen is changed into determination of freezing surface temperature range measured by a katathermometer from determination of the distance to the liquid nitrogen surface, so that the novel method is simple, rapid and economic, and ensures that different batches of frozen semen are consistent because of freezing conditions. Thus, stable preparation of high-quality frozen semen is achieved; and progress of protection work of the national treasure panda is greatly promoted.

Description

technical field [0001] The invention relates to a cryopreservation of captive animal semen, in particular to a new method for preparing giant panda frozen semen. Background technique [0002] Giant panda frozen semen technology was first established by Chengdu Zoo in 1980. At that time, the preparation technology of granule frozen semen was used. The method used was borrowed from the preparation technology of bovine frozen semen. As a result, the quality of frozen semen was poor, the repeatability was poor, and it could not be directly labeled. and other issues, this method has been eliminated now. Since the end of the 1990s, the frozen sperm of giant pandas has been gradually applied to the breeding of giant pandas. The frozen sperm of giant pandas usually adopts manual freezing, program cooling and freezing in liquid nitrogen transport tanks. Preparation method, the details are as follows: [0003] (1) Manual freezing method [0004] "Research on Electric Stimulation Spe...

AI Technical Summary

Problems solved by technology

The main disadvantage is the limited amount of frozen semen that can be prepared

[0011] Among the above three preparation methods of giant panda straw frozen semen, the first method, the manual freezing method, has been widely used and reported in production because of its simple operation, convenience, quickness, low liquid nitrogen consumption, and good effect. There are some problems: the main problem is that the quality of frozen semen prepared by this method is poor in repeatability between different production batches. The height from the surface to the freezer shelf is a very rough indicator, which is difficult to grasp accurately, resulting in the fact that the temperature of the frozen surface is actually uncertain each time the semen is frozen, and the uncertain temperature of the frozen surface can directly lead to the freezing of different batches of semen The difference in speed is large, and the difference in freezing speed can directly affect the quality of frozen semen, which is an important reason for the unstable quality of frozen semen produced by the reported giant panda thin tube frozen semen

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