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Method for harvesting dunaliella salina

A harvesting method and salina technology, applied in the field of microalgae, can solve the problems of high equipment cost, high energy consumption, product loss and the like

Inactive Publication Date: 2013-05-15
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above-mentioned methods all have the disadvantages of high equipment cost, high energy consumption, and easy product loss, and all of them are harvesting methods for the purpose of extracting natural β-carotene

Method used

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  • Method for harvesting dunaliella salina
  • Method for harvesting dunaliella salina
  • Method for harvesting dunaliella salina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] After 20 days of winter salina culture, the algal cell density reached 3.32×10 6 cells / mL, the salinity of the algae solution is 134‰, the temperature of the algae solution is 15°C, and the pH is 8.2. Add PAC to reach a final concentration of 100mg / L. After stirring evenly, let stand for 41 h and then remove the supernatant. The algae mud was collected and the flocculation efficiency was 92.2%.

Embodiment 2

[0029] After 20 days of winter salina culture, the algal cell density reached 5.18×10 6 cells / mL, the salinity of the algae solution is 107‰, the temperature of the algae solution is 15°C, and the pH is 7.6. Add PAC to reach a final concentration of 150mg / L. After stirring well, let it stand for 24 hours and then remove the supernatant. The algae mud was collected and the flocculation efficiency was 93.6%.

Embodiment 3

[0031] After 12 days of summer salina cultured, the algal cell density reached 2.75×10 6 cells / mL, the salinity of the algae solution is 220‰, the temperature of the algae solution is 30°C, and the pH is 8.5. Add PAC to reach a final concentration of 130mg / L. After stirring well, let it stand for 24 hours and then remove the supernatant. The algae mud was collected and the flocculation efficiency was 90.1%.

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Abstract

The invention discloses a method for harvesting dunaliella salina, relates to a microalgae and provides a dunaliella salina harvesting method which is higher in efficiency and lower in energy consumption. The method comprises the steps of 1) at the later period of dunaliella salina cultivation, harvesting when the algae cell density is more than (1-10)*106 / mL; and 2) adding flocculant mother liquid into algae liquid, uniformly stirring, standing, removing the supernatant, and collecting to obtain algae paste, and calculating the flocculation efficiency. The inorganic flocculant is adopted to efficiently flocculate and enrich the dunaliella salina in a high-salinity culture solution. The use amount of the flocculant is determined according the temperature, the salinity, the pH and the cell concentration of the algae liquid, the least use amount cannot be less than 10mg / L, and the largest use amount cannot be more than 2000mg / L. The method is simple and economic, and the inorganic flocculant is utilized, that is, dunaliella salina cells and the flocculant are mutually absorbed, agglomerated, flocculated and deposited under the action of electrostatic forces, intermolecular gravitation, hydrogen bonds and the like, thereby achieving the purposes of separating the algae cells from the culture mediumand and enriching the algae cells. The flocculant is independently added, so that the cost is low, flocculating constituent is formed rapidly, and the deposition speed is high.

Description

technical field [0001] The invention relates to a microalgae, in particular to a method for harvesting salina. Background technique [0002] It is an indisputable fact that fossil fuels will eventually be exhausted and the pollution caused by them will become increasingly serious. Finding alternative energy sources has become a common issue around the world. Due to its low-carbon, renewable and other advantages, biomass energy has become a strong competitor of alternative energy sources, which has aroused great interest of scientific researchers. Salina, as a raw material for biomass energy, has the outstanding advantages of fast growth, easy cultivation, high lipid content, and does not compete with people for food, land, or water with farmers. [0003] Flocculation and sedimentation is a traditional microalgae separation method, which is low in cost, easy to operate and high in recovery. Zhou Quan ([1] Zhou Quan, Research on culture of salt algae and air flotation harves...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 陈长平钱领刘广发梁君荣高宇高亚辉
Owner XIAMEN UNIV
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