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Method for detecting content of manganese in sewage by using fluorescent bacteria

A technology of fluorescent bacteria and manganese content, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of heavy workload, expensive equipment, complicated processing, etc., and achieve the effect of low cost, intuitive detection method, and simple operation of growth and cultivation

Inactive Publication Date: 2013-05-08
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problems of expensive equipment, complex processing, high cost, heavy workload and large error in the existing method for detecting the manganese content of sewage, and provides a method for detecting the manganese content of sewage by using fluorescent bacteria method

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  • Method for detecting content of manganese in sewage by using fluorescent bacteria

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specific Embodiment approach 1

[0020] Specific embodiment 1: This embodiment provides a method for detecting the manganese content of sewage by using fluorescent bacteria, which is specifically completed according to the following steps:

[0021] 1. Configure as LB liquid medium: add yeast extract, peptone and NaCl to water and mix, and sterilize at 110°C~130°C for 10min~30min to obtain LB liquid medium; wherein, the mass ratio of peptone to yeast extract (1.5~2.5):1, the mass ratio of NaCl to yeast extract is (1.5~2.5):1, the ratio of the volume of water to the mass of yeast extract is (0.8L~1.2L): 1g;

[0022] 2. Weigh the LB liquid culture medium obtained in step 1, add fluorescent bacteria to it, shake and culture at 50r / min~70r / min at a temperature of 36°C~38°C for 8h~12h, and obtain plateau bacteria solution; wherein, the mass ratio of the volume of fluorescent bacteria added to the yeast extract in step 1 is (1mL~2mL): 1g;

[0023] 3. Take the LB liquid culture medium obtained in step 1, add the pl...

specific Embodiment approach 2

[0033] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the mass ratio of peptone and yeast extract in the described step 1 is 2:1, the mass ratio of NaCl and yeast extract is 2:1, the mass ratio of water The ratio of the volume to the mass of the yeast extract is 1L:1g. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0034] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in Step 1, sterilization is carried out at 120° C. for 20 minutes. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a method for detecting the content of manganese in sewage by using fluorescent bacteria, relating to the field of methods for detecting the content of manganese in the sewage and aiming to solve the problems that the traditional method for detecting the content of manganese in the sewage is expensive in equipment, complex in processing, high in cost, large in workload and high in error. The method for detecting the content of manganese in the sewage by using the fluorescent bacteria comprises the steps of: 1, preparing an LB (Luria-Bertani) liquid culture medium; 2, culturing fluorescent bacteria; 3, diluting a bacterial solution; 4, preparing bacterial solutions with different manganese contents and culturing; 5, carrying out fluorescent detection; 6, establishing a rectangular coordinate system and calculating a regression equation; 7, detecting the fluorescent intensity of the bacteria solution containing the sewage to be detected; and 8, working out the manganese content of the sewage to be detected. The method disclosed by the invention is applied to the field of sewage detection.

Description

technical field [0001] The present invention relates to the field of methods for detecting the manganese content of sewage. Background technique [0002] Manganese (Mn) is widely distributed in the ecosystem and is one of the essential trace elements for the body. The average content of manganese in the earth's crust is 1000mg / kg. Manganese pollution in the environment will cause the water body to have a large color and a high fishy smell. Excessive manganese content will affect the normal growth and development of living organisms and even cause death. Excessive intake of manganese can cause certain organ lesions and cause chronic poisoning to the human body. Drinking water with high manganese content for a long time can cause diseases such as loss of appetite, vomiting, diarrhea, gastrointestinal disorders, and nervous system damage. With the development of industry, industry, agriculture, transportation and medicine, especially in recent years, with the wide applicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 王云彪侯晓丽张凤君
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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