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Bacillus amyloliquefaciens and application thereof

A technology of amyloliquefaciens and bacilli, applied in the field of microorganisms

Active Publication Date: 2014-08-20
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus amyloliquefaciens is widely distributed in nature, but there is no report of Bacillus amyloliquefaciens controlling cabbage wilt

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1. Isolation and strain identification of Bacillus amyloliquefaciens MH71 of the present invention

[0074] 1. Sample collection

[0075] Permafrost samples collected from the coldest place in the northernmost part of China - Anji Village, Mohe, Heilongjiang.

[0076] 2. Isolation and screening of strains and antagonistic screening

[0077] The conventional soil samples were separated by gradient dilution coating, cultured at 28°C with 5 kinds of media including LB, NA, KB, PYG and CM0002, and the bacteria with large differences in colony morphology were picked and cultured in LB. Based on purification and preservation, the primary screening and multiple re-screening of antagonistic bacteria were carried out with Fusarium wilt as the target pathogen, and finally a bacterial strain with strong antibacterial activity was obtained, which was named MH71.

[0078] 3. Identification of strains

[0079] Morphological, physiological and biochemical characteristics an...

Embodiment 2

[0086] Example 2, Determination of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MH71 inhibition spectrum of the present invention

[0087] Pathogens tested:

[0088] Fusarium oxysporum Schl.f.sp. conglutinans (Wollenw.) Snyder&Hansen];

[0089] Watermelon Fusarium wilt (Fusarium oxysporumf.sp.niveum);

[0090] Pea Fusarium root rot - Fusarium solanif.sp.pisi;

[0091] Fusarium oxysporum Schlecht.;

[0092] Rhizoctonia cereali;

[0093] Wheat take-all (Gaeumannomyces graminis (Sacc.) Arx & Olivier var. tritici J. Walker);

[0094] Botrytis cinerea Per. ex Fr. Botrytis cinerea Per. ex Fr.

[0095] Strawberry gray mold (Botrytis cinerea Persoon);

[0096] Botrytis cinerea Pers. Botrytis cinerea Pers.

[0097] Peach brown rot fungus——Monilinia fracticola (wint.) Rehm);

[0098] Capsicum anthracnose (Colletotrichum capsici);

[0099] Grape anthracnose bacteria - Colletortrichum gloeosporioides Penz. e t Sacc.);

[0100] Pleurotus ostreatus Pseudomonas tollasii ...

Embodiment 3

[0110] Example 3, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MH71 of the present invention and biocontrol-related functional detection

[0111] Siderophore Detection Medium for siderophore detection (CAS medium) (Schwyn B. and Neilands J.B. Universal chemical assay for the detection and determination of siderophores [J]. Analytical Biochemistry, 1987, 160: 47-56): by Composed of 4 solutions (solutions 1-3 and casamimo acid), sterilized separately before mixing. Solution 1CAS / HDTMA: ① CAS solution: 60.5mg CAS (chrome azure) dissolved in 50ml water; ② Iron solution: 1Mm FeCl 3 ·6H 2 O was dissolved in 10mM HCl, pH 2.0; ③HDTMA solution: 72.9mg hexadecyltrimethylammonium bromide was dissolved in 40ml water. Mix the solution ① with 10ml of the solution ②, add it into the solution ③ and stir evenly, and sterilize the obtained blue-black liquid, which is the CAS / HDTMA solution. Solution 2.Salts / Buffer solution: ①Salts (750ml): K H 2 PO 4 0.3g, NaCl0.5g, NH 4 Cl1.0g...

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Abstract

The invention discloses bacillus amyloliquefaciens and application thereof. The strain number of the bacillus amyloliquefaciens is MH71, and the register number of the bacillus amyloliquefaciens in the China General Microbiological Culture Collection Center is CGMCC No.6978. The bacillus amyloliquefaciens has obvious inhibition effect on multiple pathogenic fungi such as botrytis cinerea, fusarium disease, brown rot, root rot and colletotrichum acutatum on fruits and vegetables and pathogenic bacteria such as black rot of Chinese cabbage, brown blotch diseases of oyster mushroom and angular leaf spot of cucumber. The bacillus amyloliquefaciens MH71 is fast in breeding speed, can be artificially cultured and has strong stress resistance capacity and important significance for prevention and treatment of soil-borne diseases such as cabbage wilt disease caused by fungi.

Description

technical field [0001] The invention relates to a bacillus amyloliquefaciens and application thereof, belonging to the field of microorganisms. Background technique [0002] Plant fungal diseases have brought huge losses to agricultural production, and plant soil-borne fungal diseases are an important class of difficult-to-control diseases, which have seriously affected modern agricultural production, especially vegetable production in protected areas. In recent years, facility vegetables have developed rapidly, and the special conditions of facility cultivation have led to serious soil-borne diseases of various crops in production, posing a serious threat to vegetable production in protected areas, among which Fusarium wilt caused by Fusarium oxysporum is an important Fungal diseases, cabbage wilt, foreign reports that cabbage wilt is caused by Fusarium oxysporumf.sp.conglutinans (Ramirez-Villupadua J, Endo R M, Bosland P, et al.. A new race ofFusarium oxysporumf.sp.conglu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/42C12N9/54C12P1/04A01N63/00A01N63/02A01P1/00A01P3/00C12R1/07
Inventor 卢彩鸽刘伟成张殿朋吴慧玲刘霆董丹张涛涛刘德文卢向阳田兆丰
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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