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Nucleic acid purifying column system and its application in nucleic acid extraction

A purification column and nucleic acid technology, which is applied in the nucleic acid purification column system and the application field of nucleic acid extraction. It can solve the problems that cannot be used in combination with each other, the conversion steps of different samples are complicated, and cannot meet the continuous operation of nucleic acid extraction, etc., to achieve continuous operation. Requirements, reduce the steps of removing impurities, and ensure the effect of stability

Active Publication Date: 2013-05-08
HANGZHOU KMB BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing nucleic acid extraction technology is affected by the structure of the preparative column itself. After each elution or centrifugation, the preparative column must be re-placed in a new centrifuge tube, so the conversion steps between different samples are complicated.
The existing preparative columns cannot be used in combination with each other, so they cannot meet the continuous operation of nucleic acid extraction

Method used

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  • Nucleic acid purifying column system and its application in nucleic acid extraction
  • Nucleic acid purifying column system and its application in nucleic acid extraction
  • Nucleic acid purifying column system and its application in nucleic acid extraction

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preparation example Construction

[0041] The preparation column of the present invention is made of medical-grade polypropylene, and the production process is strictly controlled without DNA, DNase, RNase and other pollution.

[0042] The nucleic acid purification column system of the present invention can be used for extracting nucleic acid by a continuous negative pressure pumping method.

Embodiment 1

[0043] Example 1 A large number of extractions of high-purity plasmid DNA

[0044] 1. Materials and Reagents

[0045] The nucleic acid purification column system is composed of a small-volume preparative column with a diameter suitable for a 15mL centrifuge tube and a large-volume preparative column with a diameter suitable for a 50mL centrifuge tube;

[0046] Silica gel membrane: can specifically adsorb nucleic acid;

[0047] Filter membrane: The filter membrane will not specifically bind to the plasmid DNA, and its pore size can allow the plasmid DNA to pass through smoothly, while proteins, bacteria and other substances cannot pass through the filter membrane;

[0048] Centrifuge tube.

[0049] Solution I: bacterial suspension, including 2mmol / L KH 2 PO 4 , 10mmol / L Na 2 HPO 4 , 137mmol / L NaCl, 2.7mmol / L KCl, 1% Tween20, pH6.0-8.0, after adding RNase A, mix well, store at 4°C;

[0050] Solution II: Bacterial lysate, including 0.1-2.5mol / L NaOH, 0.05%-1% SDS, sealed a...

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Abstract

The invention provides a nucleic acid purifying column system and its application in nucleic acid extraction. The nucleic acid purifying column system comprises two mutually-independent biological sample preparation columns which can be overlapped up and down, each of the biological sample preparation columns comprises a body, the upper portion of the body has a sample injection port, the lower portion of the body has a sample outlet mouthpiece, the portion of each of the biological sample preparation columns above the sample injection ports is provided with a retaining component, and the lower preparation of the upper preparation column is tightly buckled in the retaining component of lower preparation column. The adoption of the mutually-overlapped purifying column system satisfies the continuous operation of experiments and the conversion between / among different samples.

Description

technical field [0001] The invention relates to the field of biological sample extraction, in particular to a nucleic acid purification column system and the application of the system in nucleic acid extraction. Background technique [0002] In the field of biology, it is often necessary to filter and purify samples. Especially in the field of molecular biology, it is often necessary to extract DNA, RNA and other macromolecular substances in biological samples, and to separate and purify these macromolecular substances. Efficient separation and purification of nucleic acid substances has become a key technology in the field of molecular biology. Separating and extracting nucleic acid from biological samples using preparative columns has become a common method in the field of molecular biology. The usual preparative column is a circular tubular structure, including a circular tube column, one end of the column has a sealed cover, and the other end is filled with a porous si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12N15/10
Inventor 林源吉吕航
Owner HANGZHOU KMB BIOTECH
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