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Phosphatidylcholine high-temperature purification method

A technology for phosphatidylcholine and high-temperature purification, applied in the field of phospholipids, can solve the problems of difficulty in separation and purification of phosphatidylcholine, inconvenience in large-scale production and the like

Inactive Publication Date: 2013-05-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soybean phospholipid is a mixture of various phospholipids. In addition to phosphatidylcholine, soybean alcohol-soluble phospholipid also contains a certain amount of other phospholipids such as phosphatidylethanolamine. Due to the similar molecular structure of these phospholipids, the separation of phosphatidylcholine with higher purity Purification becomes more difficult
At present, the solvent fractionation method can be used in industry to prepare products with a PC content of about 70%. Most of the preparation of higher-purity phosphatidylcholine adopts the method of column chromatography, which is not convenient for large-scale production.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0014] Accurately weigh 10 grams of alcohol-soluble phospholipid raw material, heat it at 100°C for 1 hour, dissolve it in 12 ml of n-hexane after cooling, vacuum rotary evaporation and thermalization in an oil bath at 180°C for 0.5 hour to obtain black phospholipid thermal product, phospholipid Add acetone to the heating material and wash until free of oil. The residue was dissolved in 20 ml of 85% ethanol, stirred for 10 minutes, and then centrifuged at 4500 rpm for 30 minutes to obtain ethanol-soluble matter. The ethanol soluble matter was added to a Buchner funnel covered with 10 g of silica gel, and eluted with 85% ethanol which was 5 times of the ethanol soluble matter, the ethanol solution was collected, and the ethanol was removed in vacuum to obtain a product with a phosphatidylcholine content of 90.01%.

Embodiment 2

[0016] Accurately weigh 10 grams of alcohol-soluble phospholipid raw material, heat it at 100°C for 1 hour, dissolve it in 12 ml of n-hexane after cooling, and vacuum rotary evaporation and thermalization in an oil bath at 180°C for 1 hour to obtain black phospholipid thermal product; phospholipid Add acetone to the heating material and wash until free of oil. The residue was dissolved in 20 ml of 85% ethanol, stirred for 10 minutes, and then centrifuged at 4500 rpm for 30 minutes to obtain ethanol-soluble matter. The ethanol soluble matter was added to a Buchner funnel covered with 20 grams of silica gel, and eluted with 85% ethanol which was 10 times of the ethanol soluble matter, and the ethanol solution was collected, and the ethanol was removed in vacuum to obtain a product with a phosphatidylcholine content of 88.6%.

Embodiment 3

[0018] Accurately weigh 10 grams of alcohol-soluble phospholipid raw material, heat it at 100°C for 1 hour, dissolve it in 12 ml of n-hexane after cooling, vacuum rotary evaporation and thermalization in an oil bath at 180°C for 0.5 hour to obtain black phospholipid thermal product; phospholipid Add acetone to the heating material and wash until free of oil. The residue was dissolved in 20 ml of 85% ethanol, stirred for 10 minutes, and then centrifuged at 4500 rpm for 30 minutes to obtain ethanol-soluble matter. The ethanol soluble matter was added to a Buchner funnel covered with 20 grams of silica gel, and eluted with 85% ethanol which was 5 times of the ethanol soluble matter, the ethanol solution was collected, and the ethanol was removed in vacuum to obtain a product with a phosphatidylcholine content of 92.1%.

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Abstract

The invention relates to a phosphatidylcholine high-temperature purification method which belongs to a phospholipid technical field. According to the invention, alcohol-soluble phospholipid with a phosphatidylcholine content higher than 45% is adopted as a raw material, and is heated for 1h under a temperature of 100 DEG C; the material is cooled, and is dissolved in 12ml of n-hexane; the mixture is subjected to vacuum rotation evaporation desolvation and thermalization under an oil bath with a temperature of 180 DEG C, such that a black phospholipid thermalization product is obtained; acetone is added into the phospholipid thermalization product for washing, until the phospholipid thermalization product is oil-free; residual is dissolved in 85% ethanol, and the mixture is stirred for 10min; the mixture is subjected to centrifugation for 30min under a rotation speed of 4500rpm, such that an ethanol-soluble substance is obtained; the ethanol-soluble substance is added into a Buchner funnel paved with silica gel, and is eluted by using 85% ethanol; ethanol solution is collected; and vacuum ethanol removing is carried out, such that a product with a phosphatidylcholine content of approximately 90% is obtained.

Description

technical field [0001] A method for purifying phosphatidylcholine at high temperature belongs to the technical field of phospholipids. technical background [0002] Soybean phosphatidylcholine (PC) with higher purity and high purity is effective for modern diseases such as cardiovascular disease, geriatric disease and improving children's intelligence in modern society due to changes in dietary structure, refinement of food processing and modern social environment. The biological function of phospholipids is beyond the reach of ordinary phospholipids, so it has attracted the attention of countries all over the world. In modern society, phospholipids, mainly PC, are known as brain food, blood vessel scavenger, edible cosmetics, cell protector, longevity factor, etc. Soybean phospholipid is a mixture of various phospholipids. In addition to phosphatidylcholine, soybean alcohol-soluble phospholipids also contain a certain amount of other phospholipids such as phosphatidylethan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10
Inventor 史苏佳曹栋曹沛
Owner JIANGNAN UNIV
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