Raw milk somatic cell test paper and preparation method thereof

A technology for detecting test strips and somatic cells, which is applied in the preparation of test samples, analysis by making materials undergo chemical reactions, and material analysis by observing the impact on chemical indicators. It can solve cumbersome operations, long detection time, Poor detection accuracy and other problems, to achieve the effect of obvious color change, fast detection speed and low detection cost

Active Publication Date: 2014-12-24
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing methods have the following problems: expensive instruments and equipment, high operating costs; poor detection accuracy; long detection time; or cumbersome operations that require professional technicians to operate
Due to the limitation of detection technology and cost, my country's dairy industry and dairy cattle breeding industry seldom detect the SCC of milk, and only a few intensively managed pastures use the somatic cell detector produced by Danish FOSS company for monthly inspection, but the detection cost is expensive
[0007] With the continuous development of the dairy industry, the detection of somatic cells will gradually become a daily task. However, the existing methods for detecting the number of somatic cells in raw milk are time-consuming, cumbersome, and require professional technicians to operate. High cost and inaccurate problems

Method used

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  • Raw milk somatic cell test paper and preparation method thereof
  • Raw milk somatic cell test paper and preparation method thereof

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Experimental program
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Embodiment 1

[0036] This embodiment provides a raw bovine milk somatic cell detection test paper, which is prepared by the following method:

[0037] 1. Pretreatment: Place the filter paper in a copper sulfate drying dish for 20-40 minutes; the size of the filter paper in my actual experiment is 10cm 2

[0038] 2. The first soaking: soak the pretreated filter paper in EDTA solution with a concentration of 0.09-0.12wt% for 2 minutes, and then soak the filter paper in 0.1mol / L Tris-hydrochloric acid buffer for 2 minutes to make the first time The pH of the soaked filter paper is 7.0-8.0;

[0039] 3. First drying: Put the filter paper soaked for the first time in a hot air drying box and dry it at 50-90℃;

[0040] 4. The second soaking: soak the filter paper after the first drying in the second soaking solution for 1-5 min;

[0041] Wherein, the second soaking liquid is prepared by the following method:

[0042] Dissolve 1 part by weight of 1-amino-2-naphthol-4-sulfonic acid in 6 parts by weight of wat...

Embodiment 2

[0048] This embodiment provides a raw bovine milk somatic cell detection test paper, which is prepared by the following method:

[0049] 1. Pretreatment: Place the filter paper in a copper sulfate drying dish for 20-40 minutes;

[0050] 2. The first soaking: soak the pretreated filter paper in EDTA solution with a concentration of 0.05-0.15wt% for 2 minutes, and then soak the filter paper in 0.5mol / L Tris-hydrochloric acid buffer for 2 minutes to make the first time The pH of the soaked filter paper is 7.0-8.0;

[0051] 3. First drying: Put the filter paper soaked for the first time in a hot air drying box and dry it at 50-90℃;

[0052] 4. The second soaking: soak the filter paper after the first drying in the second soaking solution for 1-5 min;

[0053] Wherein, the second soaking liquid is prepared by the following method:

[0054] Dissolve 3 parts by weight of 1-amino-2-naphthol-4-sulfonic acid in 18 parts by weight of water and stir it into a paste to form a paste of 1-amino-2-naph...

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Abstract

The invention relates to raw milk somatic cell test paper and a preparation method of the raw milk somatic cell test paper. The preparation method of the raw milk somatic cell test paper comprises soaking filter paper in a surfactant solution for 1-10min, and soaking the filter paper in a buffer solution for 1-10min in first soaking, drying the filter paper for the first time, soaking the filter paper soaked in the first soaking in a second soaking solution for 1-5min in second soaking, drying the filter paper for the second time, and obtaining the raw milk somatic cell test paper. The raw milk somatic cell test paper has the advantages of being simple in operation when in use, capable of economically and accurately being used for daily monitoring of mastitis of dairy cattle and daily monitoring of raw milk somatic cell indexes.

Description

Technical field [0001] The invention relates to a raw cow milk somatic cell detection test paper and a preparation method thereof, and belongs to the technical field of food detection. Background technique [0002] Somatic Cell Count (SCC) in milk refers to the total number of cells per milliliter of milk. Most of the somatic cells are white blood cells (ie macrophages, neutrophils and lymphocytes), which account for more than 95% of the somatic cells, and the rest are shed epithelial cells. SCC produced from healthy breast milk is usually less than 100,000 / mL, including 1-7% epithelial cells and 30-74% macrophages. [0003] There are many factors that affect milk SCC. Generally speaking, microorganisms are the main reason for inducing mastitis and increasing SCC. 95% of mastitis is caused by Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli, etc. Secondly, when breast inflammation occurs due to trauma or disease, it can also cause a large increase in SCC. There...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N1/38
Inventor 富鑫
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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