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Application of beta-catenin and gene

A catenin and gene technology, which is applied in the determination/inspection of microorganisms, material inspection products, biochemical equipment and methods, etc., can solve problems such as unclear mechanisms and achieve accurate diagnosis

Inactive Publication Date: 2013-04-24
SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the binding sites of JunD and MLL are the same, the regulatory effects of menin on the two are completely opposite, and the mechanism is still unclear

Method used

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  • Application of beta-catenin and gene
  • Application of beta-catenin and gene
  • Application of beta-catenin and gene

Examples

Experimental program
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Effect test

Embodiment 1

[0021] 1. Establishment of transgenic mouse model

[0022] Establishment of islet β cell conditional knockout β-catenin gene (Catnb) mice and Men1 / β-catenin double knockout mouse models by Cre / loxP technology:

[0023] 1) Homozygous Catnb purchased from Jackson Lab, USA lox / lox Mice (carrying loxP-modified β-catenin gene), which were mated with wild-type C57 mice to obtain Catnb lox / + heterozygous mice.

[0024] 2) Combine β cell-specific Rat-insulin-promoter Cre (Rip-Cre) mice with Catnb lox / lox Mice were mated to obtain β-cell conditional knockout of β-catenin gene mouse model (Catnb lox / lox -RipCre+) (hereinafter referred to as BBKO mice).

[0025] 3) Men1 conditional knockout mice (Men1 lox / lox -RipCre + ) (hereinafter referred to as Menin-KO mice) and Catnb lox / lox The mice were mated, and a homozygous double gene knockout mouse model (Catnb lox / lox -Men1 lox / lox -RipCre + ) (hereinafter referred to as DBKO mice)

[0026] The environment in which the mice were ...

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Abstract

The invention belongs to the field of biological medicine, and in particular relates to an application of beta-catenin and gene in a reagent for detecting insulinoma and an application of the beta-catenin and gene in drug screening of insulinoma drugs. Through the experiments of dissecting the remain of a transgenic mice, detecting the blood sugar and fasting insulin level, the influence relation of the beta-catenin to the generation of the insulinoma is researched. The experiment result shows that the beta-catenin and the insulinoma are in positive relation, once the lack of beta-catenin, the occurrence rate of the islet cell tumor is obviously reduced. The application provided by the invention has the beneficial effects that the mice with insulinoma can be fast and accurately diagnosed through the detection of beta-catenin, and the experimental data and theoretical basis are provided for the application of beta-catenin in the reagent for detecting the insulinoma and in the drug screening of the insulinoma drug in the clinical diagnosis.

Description

technical field [0001] The invention belongs to the field of biomedicine, and particularly relates to the application of beta-catenin and gene in detecting insulinoma reagents and screening insulinoma drugs. Background technique [0002] Insulinoma is the most common functional islet cell tumor, which was first reported by Nicholis in 1902 and found in autopsy, with an incidence of about 1 / 250,000. The etiology of insulinoma is still unclear, and its typical manifestations are obvious hypoglycemia and neurological symptoms. Due to the low incidence and limited knowledge of community doctors, it is easy to be misdiagnosed as various neurological diseases, including epilepsy, hysteria, and mental disorders. Wait. Long-term hypoglycemia can cause irreversible brain damage, mental decline, slow behavior, and even inability to take care of oneself in severe cases, which often brings great harm to patients. If islet cell tumors can be detected in time, quickly and accurately, so...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68
Inventor 宁光李峰李小英曹亚南姜秀丽
Owner SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES
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