Cell culture medium for passive growth of cells and preparation method of cell culture medium

Abstract

The invention relates to the filed of the biotechnology, and in particular relates to preparation of a cell culture medium suitable for the passive growth of cells. The method comprises the following step of preparing the cell culture medium for the passive growth of the cells by a DMEM (dulbecco modified eagle medium), 3g / L of L-glutamine, 1.5mg / L of hydrocortisone, fetal calf serum, penicillin and streptomycin according to a certain proportion. According to the invention, the long-term passive growth of various cells under a sealed environment can be guaranteed, the raw materials of the existing culture medium do not need to be greatly changed, the cells can be guaranteed to resist the severe environments, i.e., the low oxygen, the low temperature and the micro gravity for a long time, the proper growth of various cells can be guaranteed, and the apoptosis rate can be reduced, so that necessary conditions can be provided for the research of the long-term chronic hypoxia injury, the long-term chronic low-temperature injury and the long-term exposure in the micro gravity environment of the cells.

Description

technical field [0001] The invention relates to the preparation of a cell culture medium for long-term passive growth of various cells, which belongs to the field of biotechnology, and ensures that various cells can resist unfavorable factors in the external environment for a long time, grow moderately, and reduce the death rate, thereby providing cells with field of study provides the necessary conditions. Background technique [0002] With the advancement of science and technology, the deepening of human understanding, and the increase in research in microscopic fields, experiments in medical-related fields need to be carried out from all aspects of human body, tissue, cell, and animal experiments. As a basic technology, cell culture has gradually become a model. Generally speaking, the conditions of cell culture in the laboratory are relatively harsh. The currently recognized cell culture method is still relatively conservative, and it is necessary to strictly abide by th...

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Problems solved by technology

[0003] At present, a variety of chronic diseases seriously threaten the health and quality of life of human beings. Research on their pathogenic mechanisms is widely carried out in the field of cells. However, the existing cell culture medium needs to be replaced regularly, and the replacement interval is mostly 24 to 48 hours. , which limits the study of long-term cell damage

For example, according to the existing technical means, to study the influence of hypoxia on vascular endothelial cells for 15 days, in order to ensure the survival of the cells during the research, it is necessary to replace the fresh cell culture medium every 24 to 48 hours. A certain concentration of oxygen must be brought in, which will affect the research results to a certain extent

In addition, in some studies on space cell loading, there is no condition for regular replacement of the medium, and the existing medium cannot guarantee the long-term survival of the cells, which brings many obstacles to the research in the field of space medical cells

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