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Micro-fluidic cell culturing chip and preparation method thereof

A microfluidic chip, cell culture technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve problems such as no substantial breakthroughs, achieve good application prospects, reduce Dosage and simple operation

Inactive Publication Date: 2013-04-24
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, using a microfluidic chip to prepare microstructures and microchannels on its surface, relying on the laminar flow effect between multiple layers of liquid in the microchannel to drive the sample microfluidics, and to complete the implantation of various cells at the same time, currently in a variety of The application field of cell co-culture has not yet had a substantial breakthrough

Method used

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  • Micro-fluidic cell culturing chip and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a microfluidic chip for co-culture of two kinds of cells. Add two kinds of cell solutions from the left and right cell solution adding areas, control the liquid difference height between the left and right cell solution adding areas and the cell solution storage area, so as...

Embodiment 2

[0027] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a multi-cell co-cultured microfluidic chip. Add two kinds of cell solutions from the left and right cell solution adding areas, control the liquid difference height between the left and right cell solution adding areas and the cell solution storage area, so as to control th...

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Abstract

The invention relates to a micro-fluidic cell culturing chip and a preparation method thereof. A microstructure and a micro-channel are arranged on the surface of a micro-fluidic chip; two cells are respectively fixed in specific cell culturing regions; signal transmission and interaction between the cells are carried out between the cells through the micro-channel; under a microscope, the interaction between the two cells in the growing process can be intuitively studied and metabolites in the cell growing process are analyzed; a new idea and a new research technique are provided for researches on the cell biology, and the micro-fluidic cell culturing chip is mainly applied to the related field of cell biology, genetics, drug screening and the like. Through adoption of the micro-fluidic chip, co-culturing of the two cells is achieved, the operation is simple, parallel implantation and co-culturing of the two cells are realized, the usage amounts of reagents and samples are reduced, the cell implanting process is simplified, the interaction between the two cells in the growing process can be intuitively studied under the microscope, the metabolites in the cell growing process is analyzed and a brand new analysis technology is provided for researches on the cell biology and the micro-fluidic cell culturing chip provided by the invention is portable, economic, rapid, efficient and accurate.

Description

technical field [0001] The invention relates to a microfluidic cell culture chip and a preparation method thereof. The surface of the microfluidic chip has microstructures and microchannels. Two kinds of cells are respectively fixed in their specific cell culture areas, and the cells are separated by microchannels. Under the microscope, the interaction between the two types of cells during the growth process can be visually studied, and the metabolites during the cell growth process can be analyzed, which provides new ideas and new research techniques for the study of cell biology. It is mainly used in related fields such as cell biology, genetics and drug screening. Background technique [0002] In the late 1980s, in order to establish a culture system more similar to the in vivo environment and make the in vitro environment as consistent as possible with the in vivo environment, so that cells can communicate with each other and support each other for growth and proliferati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 叶嘉明王晓东沙俊聂富强
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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