Production process of composite probiotics
A technology of compound probiotics and production process, which is applied in application, animal feed, animal feed, etc., can solve the problems of short shelf life, evolution of probiotic population structure, low biological activity, etc., achieve good antibacterial effect, prevent pollution, good Bacteriostatic effect
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Embodiment 1
[0057] In Example 1, the specific process steps are as follows:
[0058] (1) Breeding:
[0059] ①Add 700g of barley germ, 600g of peptone, 1000g of agar, 300g of sodium citrate, 150g of potassium dihydrogen phosphate, 7.5g of magnesium sulfate, and 200g of sodium bicarbonate into the batching tank, make up to 100L of distilled water, and adjust the pH after heating to boiling Transfer to the saccharification tank after 4.5, add 2.1×10 to the saccharification tank 5 U saccharification enzyme, heat preservation and saccharification at 60°C for 2h to obtain 104.6kg saccharification liquid
[0060] ②Transfer 104.6kg of saccharification liquid into the fluid heater, heat up to 95℃ within 5s, keep it for 1h, and sterilize at high temperature. The sterilized saccharification liquid flows through the cooling pipe, and the temperature is reduced to 30℃ within 5s to obtain 103.8kg breeding Culture medium
[0061] ③Equally distribute 103.9kg of breeding medium to four chemostats, and use arrow...
Embodiment 2
[0071] In Example 2, the specific process steps are as follows:
[0072] (1) Breeding:
[0073] ①Add 2.4kg of barley germ, 1.6kg of peptone, 2.4kg of agar, 0.8kg of sodium citrate, 0.36kg of potassium dihydrogen phosphate, 0.019kg of magnesium sulfate, and 0.6kg of sodium bicarbonate into the batching tank, make up to 200L of distilled water, and heat to After boiling, adjust the pH to 5.0 and then transfer to the saccharification tank, and add 9.6×10 to the saccharification tank 5 U saccharification enzyme was incubated at 60°C for 4 hours to obtain 212.44kg saccharification liquid;
[0074] ②Transfer 212.44kg of saccharification liquid into the fluid heater, heat up to 100℃ within 8s, keep it for 1.5h, and sterilize at high temperature. The sterilized saccharification liquid flows through the cooling pipe and is cooled to 40℃ within 8s to obtain 210.18kg Breeding medium
[0075] ③Distribute 210.18kg of breeding medium evenly into four chemostats, and use arrow inoculation machine ...
Embodiment 3
[0085] In Example 3, the specific process steps are as follows:
[0086] (1) Breeding:
[0087] ①Put 2.85kg barley germ, 2.1kg peptone, 3.3kg agar, 1.05kg sodium citrate, 0.495kg potassium dihydrogen phosphate, 0.0255kg magnesium sulfate, 0.75kg sodium bicarbonate into the batching tank, make up to 300L with distilled water, and heat to After boiling, adjust the pH to 4.8 and then transfer to the saccharification tank, add 9.98×10 to the saccharification tank 5 U saccharification enzyme was incubated at 60°C for 3h to obtain 311.9kg saccharification liquid;
[0088] ②Transfer 311.9kg of saccharification liquid into the fluid heater, heat up to 98°C within 6s, keep the temperature for 1.3h, and sterilize at high temperature. The sterilized saccharification liquid flows through the cooling pipe and is cooled to 35°C within 7s to obtain 310.08kg. Breeding medium
[0089] ③Equally distribute 310.08kg of breeding medium to four chemostats, and use arrow-type inoculation machine for the s...
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