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Down syndrome 21 chromosome miRNA differential expression map model, modeling method and application

A Down syndrome and differential expression technology, applied in the field of Down syndrome chromosome 21 miRNA differential expression map model and construction, can solve the problem of disrupting normal development, disordered diploid gene expression, and unclear clinical harmful phenotypes, etc. question

Active Publication Date: 2013-04-17
徐勇
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Problems solved by technology

However, how the 21st chromosome 3 gene leads to disordered diploid gene expression, disrupts normal development, and produces various clinically harmful phenotypes is still unclear

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  • Down syndrome 21 chromosome miRNA differential expression map model, modeling method and application
  • Down syndrome 21 chromosome miRNA differential expression map model, modeling method and application
  • Down syndrome 21 chromosome miRNA differential expression map model, modeling method and application

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Embodiment Construction

[0019] The miRNA differential expression map model related to chromosome 21 of Down's syndrome, its application and construction method will be further described in detail below mainly in conjunction with the accompanying drawings and specific examples.

[0020] The construction method, results and related detection results of an embodiment are as follows:

[0021] 1. Build

[0022] 1.1. Sample collection and collection and storage of fetal umbilical cord blood

[0023] The umbilical cord blood samples were provided by the Genetics Department of the Clinical Medical Research Center of Shenzhen People's Hospital, 6 cases of standard DS fetal umbilical cord blood (2 males, 4 females), 6 cases of normal fetal umbilical cord blood (2 males, 4 females), respectively set as DS group and healthy control group. Each sample was confirmed by conventional G-banding karyotype analysis, the gestational age was 18-22 weeks, and the pregnant women were 30-34 years old. This study was appr...

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Abstract

The expression map and the chromosome distribution characteristic of a DS (down syndrome) fetal genome-wide miRNAs are studied by an Illumina deep sequencing technology so as to obtain a down syndrome 21 chromosome-related miRNA differential expression map model, which consists of an up-regulated miRNA, four down-regulated miRNAs and 9 zero-expression miRNAs. miRNA molecules with significant difference and specific expression help to understand a molecular regulation mechanism for occurrence and development of a clinical phenotype of a DS disease and lay the foundation for further studying gene expression disorder of a DS patient genome-wide and a relationship between the gene expression disorder and relevant clinical symptoms.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a Down syndrome chromosome 21 miRNA differential expression map model and its construction method and application. Background technique [0002] Down syndrome (DS for short), also known as trisomy 21, is often accompanied by more than 80 clinical symptoms such as mental retardation, special facial features, and immune deficiency. Regarding the occurrence of various clinical symptoms in DS patients, researchers believe that it is the joint result of the disordered expression of chromosome 21 and diploid genes. However, how the 21st chromosome 3 gene leads to disordered diploid gene expression, disrupts normal development, and produces various clinically harmful phenotypes is still unclear. miRNA (microRNA, also known as microRNA), is a type of endogenous single-stranded non-coding RNA with a length of 18-24 nucleotides (nucleotides, nt for short). miRNA can specifically bind t...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
Inventor 徐勇李武县戴勇
Owner 徐勇
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