Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Peptide product for promoting calcium absorption and manufacture method thereof

A calcium absorption and peptide technology, applied in the field of peptide products, can solve the problems of complicated steps, insufficient hydrolysis reaction, and high cost

Active Publication Date: 2013-04-17
FOOD IND RES & DEV INST
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, Taiwan patent TW I298622 discloses a two-stage fermentation method for improving the nutritional efficiency of soybean protein, wherein the specification discloses a two-stage fermentation method for improving the nutritional efficiency of soybean protein, which is to pre-ferment the soybean raw material After (aspergillus fermentation) treatment, the resulting product is mixed with soybean raw materials. After adjusting the water content, lactic acid bacteria are inoculated, and the lactic acid bacteria fermentation and enzymatic hydrolysis process are continued. Finally, it can be directly used or dried and crushed to make a powder product. However, the above method needs to be cooked, and lactic acid bacteria are needed for subsequent fermentation. The steps are complicated and the cost is high. In addition, during the second stage of fermentation in this case, the moisture is controlled at 35-50%, which basically presents a solid state. state, so the hydrolysis reaction cannot be fully carried out, so it is difficult to obtain soybean protein hydrolyzate of small fragment peptides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Peptide product for promoting calcium absorption and manufacture method thereof
  • Peptide product for promoting calcium absorption and manufacture method thereof
  • Peptide product for promoting calcium absorption and manufacture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. Promoting the manufacture of calcium absorption soybean peptide

[0020]Using defatted soybean meal as raw material, inoculate Aspergillus sojae (Aspergillus sojae), wherein, the inoculum amount of Aspergillus is 0.013% of the total weight of soybean raw material, and the concentration is 0.25% by weight of CH 3 COONa / 0.25%HNaSO 3 , pH = 5.8 buffer solution to adjust the water content to 55%, and cultured for 2 days at 25-30° C. and a relative humidity of 95% or more.

[0021] Then carry out the hydrolysis reaction, mix soybean koji with water, wherein the ratio of soybean koji to water is 1:2, the hydrolysis time is 6 hours, and the hydrolysis temperature is 45°C, then increase the temperature to 90-95°C and keep it for 10 minutes. Inhibits enzyme activity.

[0022] Next, carry out the clarification treatment of the hydrolyzate, first use the press machine, put the soybean hydrolyzate in the filter bag, spread it on the press tank, and separate the solid a...

Embodiment 2

[0023] Embodiment 2. Soybean hydrolyzate inhibits the effect of calcium carbonate precipitation

[0024] According to Jin et al. (J.Agnc.Food Chem.2000) calcium carbonate crystallization experiment slightly modified, 1mL sample was mixed with 1mL 40mM calcium chloride and 1.2mL distilled water, then added 0.8mL 40mM sodium bicarbonate to react 1 hour and titrate with 0.1N NaOH to maintain pH = 8.5. The mixed solution that finishes reaction is centrifuged (centrifugal force is 2000g), gets supernatant and uses calcium measuring reagent group (Taiwan Merck company product) to measure calcium concentration, and this is soluble calcium content.

[0025] In the experiment of inhibiting calcium carbonate crystallization, the soybean protein hydrolyzate obtained by embodiment 1 has an inhibitory effect on calcium carbonate crystallization, and the evaluation of this effect is under alkaline conditions (pH=8.5), completely inhibiting the minimum sample concentration of calcium carbona...

Embodiment 3

[0026] Example 3. Effect of Soybean Hydrolyzate Inhibition

[0027] In this example, different protein sources were selected for comparison, 1 mL of sample was mixed with 1 mL of 40 mM calcium chloride and 1.2 mL of distilled water, and then 0.8 mL of 40 mM sodium bicarbonate was added to react for 1 hour, and 0.1 N hydrogen Titrate with sodium oxide to maintain pH = 8.5. The mixed solution that finishes reaction is centrifuged (centrifugal force is 2000g), gets supernatant and uses calcium measuring reagent group (Taiwan Merck company product) to measure calcium concentration, and under alkaline condition (pH=8.5), suppress carbonic acid completely The lowest sample concentration of calcium crystallization was used as an evaluation criterion for soluble calcium content.

[0028] figure 2 Inhibitory effect of calcium carbonate crystallization for different protein source substances. Among them, free amino acids such as sodium glutamate (monosodium glutamate, MSG) and glyci...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention refers to a method for manufacturing peptide product for promoting calcium absorption, comprising the steps of: mixing raw materials including soy protein with water to form a mixture including 45%-65% weight percentage water content; inoculating Aspergillus oryzae, Aspergillus sojae or their aspergillus flora into the mixture to obtain a culture, wherein an inoculum size of the aspergillus flora accounts for 0.006%-0.05% of soy protein raw materials; perform solid state fermentation on the mixture with a relative humidity of 85%-95% and a fermentation temperature between 23 and 35 DEG C to obtain a fermentation product; performing hydrolysis reaction on the fermentation product to obtain soybean hydrolysate; clarifying processing the soybean hydrolysate and collecting hydrolysate to obtain peptide products for promoting calcium absorption.

Description

technical field [0001] The present invention relates to a peptide product, in particular to a peptide product that can promote calcium absorption and a manufacturing method thereof. Background technique [0002] According to the nutritional survey of residents in Taiwan, the average calcium intake in Taiwan is 520mg for men and 542mg for women, while the average intake for developing teenagers aged 13-19 is only 51-56% of the recommended amount. Compared with the American RDA (1200mg) or RDI (1300mg) standard, it is less than half of its recommended intake. In 2002, the Department of Health revised the "Reference Intake of Dietary Nutrients for Chinese People", from the original 600 mg per day for adults to 800-1000 mg, and for teenagers to 1200 mg. [0003] Dairy products are generally familiar sources of calcium, but many people are lactose intolerant. In addition, in Taiwan, the per capita consumption of dairy products is much lower than in Western countries, which is al...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23J3/16A23J3/32A23L11/00
Inventor 庄少钧张建棣洪铭育刘毓蕙赖祥玲钱阜甯刘素娥陈怡宏程竹青
Owner FOOD IND RES & DEV INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products