Test tube one-step seedling method of taxus cuspidata

Abstract

The invention relates to a plant planting technology, namely a test tube one-step seedling method of taxus cuspidate. The test tube one-step seedling method comprises the following steps of: (1) taking a newly-sprouted main stem on a taxus cuspidate wild seedling trunk and cutting into segments as explants for further use; (2) when stem segments grow roots, utilizing an axillary bud germination growth culture medium including components and contents: a culture medium+indoleacetic acid IAA (0.03-0.04mg / L)+naphthylacetic acid NAA (0.02-0.03mg / L)+kinetin KT (0.11-0.12mg / L)+gibberellin GA3 (2.60-2.80mg / L); adding agar powder with the concentration of 8.0g / L and adding cane sugar with the concentration of 20.0g / L; adjusting the pH (Potential of Hydrogen) value to 5.6; and culturing tender stem segments under the conditions that the illumination period is 6 hours per day, the illumination intensity is 700lx and the temperature is 20+ / -2 DEG C. The invention further provides high-quality seedlings for artificially propagating and cultivating the taxus cuspidate in scales. The hardening-seedling and transplanting survival rate can reach more than 95.2%.

Description

[0001] technical field [0002] The invention relates to a plant planting technique, that is, a one-step seedling-forming method of the Northeast yew in a test tube. Background technique [0003] In the prior art, Northeast Taxus ( Taxus cuspidata Sieb. et Zucc.) also known as yew, red plate pine, Taxus yew is an evergreen tree, the number of this species is rare in the Changbai Mountains, the distribution area is very narrow, only in the Shihu Nature Reserve of Jilin Province Large population area, only sporadic distribution in other areas. It is designated as a national first-level key protected plant by the "List of National Key Protected Wild Plants (First Batch)". It is designated as a Vulnerable Species (VU) by the "Red List of Species in China". In the article "Interim Regulations on the Protection and Management of Wild Animals and Plants of Jilin Province", it is designated as a provincial first-class key protected plant. Taxus chinensis is not only a timber plan...

AI Technical Summary

Problems solved by technology

[0004] Northeast yew is usually propagated mainly by cuttings or seeds, but after the fruit matures, the seed embryo in the seed is in the late stage of the heart-shaped embryo or the early stage of the cotyledon embryo, and the seed does not germinate in the same year after sowing, and the seed coat is hard. It is difficult to break the shell and germinate after maturity, and only a few germinate after 2 to 3 years; therefore, cuttings and cuttings not only have a very low rooting rate and transplant survival rate, but also cause wild resources to be threatened by extinction

Therefore, large-scale artificial cultivation, development and utilization are greatly limited

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