Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cortex periplocae anti-tumor effective component extractive as well as preparation method and application thereof

A technology of effective components and anti-tumor drugs, which is applied in the field of anti-tumor effective component extracts of Cortex Persicae and its preparation methods and uses, can solve problems that do not involve the application of anti-tumor activities of medicinal materials, and achieve expansion of uses, Good activity and the effect of increasing added value

Inactive Publication Date: 2013-04-10
JIANGSU UNIV
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chinese patent application CN200810006406.5 "A Method for Analyzing the Chemical Components of Fatty Acids in Cortex Persicae", 200510013382.2 "Effective Parts of Cortex Perigo Cardiac in Cardiotonic Action and Preparation Method and Pharmaceutical Preparations and Uses Containing Them", are about the preparation and application of fatty acids. The cardiotonic action part of the medicinal material does not involve the application of the medicinal material in anti-tumor activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cortex periplocae anti-tumor effective component extractive as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. The preparation of the anti-tumor effective component extract of Cortex Persicae

[0023] Dry and pulverize the cypress bark, take 400g of medicinal powder, and reflux extract with 10 times the mass of water for 4 hours, repeat the extraction of the centrifuged residue once, centrifuge, combine the secondary water extracts, concentrate to 300ml, add 95% ethanol for alcohol Shen, the concentration of ethanol in the medicinal solution is about 60%, let it stand overnight, centrifuge, discard the precipitate, recover the ethanol under reduced pressure, continue to concentrate to 300ml, add an equal volume of ethyl acetate to extract 4 times, and the remaining water is reduced After the remaining ethyl acetate was removed by pressure, extract 3 times with an equal volume of water-saturated n-butanol, combine the water-saturated n-butanol extracts, recover n-butanol, and obtain 8.66 g of water-saturated n-butanol extract;

[0024] The above-mentioned n-butanol ext...

Embodiment 2

[0026] Example 2. The preparation of the anti-tumor effective component extract of Cortex Persicae

[0027] Dry and pulverize the cypress bark, take 400g of medicinal powder, reflux and extract with 10 times the mass of water for 5 hours, repeat the extraction of the centrifuged residue once, centrifuge, combine the secondary water extracts, concentrate to 300ml, add 95% ethanol for alcohol Shen, the concentration of ethanol in the medicinal solution is about 60%, let it stand overnight, centrifuge, discard the precipitate, recover the ethanol under reduced pressure, continue to concentrate to 200ml, add an equal volume of ethyl acetate to extract 3 times, and the remaining water is reduced After removing residual ethyl acetate by pressure, extract 4 times with equal volumes of water-saturated n-butanol, combine the water-saturated n-butanol extracts, reclaim n-butanol, and obtain 9.56 g of water-saturated n-butanol extract,

[0028] The above-mentioned n-butanol extract was p...

Embodiment 3

[0030] Example 3. The preparation of the extract of the anti-tumor effective part of Cortex Persicae

[0031] Dry and pulverize the cypress bark, take 400g of medicinal materials, reflux extract with 10 times the mass of 95% ethanol for 3 hours, repeat the extraction of the centrifuged residue once, centrifuge, combine the second alcohol extract, concentrate until it has no alcohol smell, add appropriate amount water, centrifuge, discard the precipitate, concentrate to about 300ml, extract 3 times with an equal volume of petroleum ether, remove the remaining petroleum ether under reduced pressure, add an equal volume of ethyl acetate to extract 4 times, and the remaining After removing residual ethyl acetate in the aqueous solution under reduced pressure, extract 4 times with an equal volume of water-saturated n-butanol, combine the water-saturated n-butanol extracts, reclaim n-butanol, and obtain 15.76 g of n-butanol extract,

[0032]Pass the n-butanol extract through a silic...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cortex periplocae anti-tumor effective component extractive, which is prepared by the following steps of: crushing cortex periplocae; extracting under heating by utilizing water at 90-100 DEG C, concentrating an extracting solution, carrying out alcohol precipitation, centrifuging, removing sediment and concentrating under reduced pressure to remove ethanol to obtain an extracting solution; or extracting under heating by utilizing ethanol with the concentration of 40-95% in a water bath of 80 DEG C, cooling and centrifuging, and concentrating to remove ethanol to obtain an extracting solution; sequentially extracting the extracting solution by utilizing ethyl acetate and water saturated n-butyl alcohol; subjecting a n-butyl alcohol extracted part to silica gel column separation, wherein a diluting agent is dichloromethane-methanol with the different volume ratios; collecting a diluting solution with the volume ratio of dichloromethane to methanol of 10: 1; and recycling an organic solvent to obtain the cortex periplocae anti-tumor effective component extractive. According to the cortex periplocae anti-tumor effective component extractive disclosed by the invention, in an in-vitro cellular toxicity test, the part has the obvious effects of inhibiting U251 (glioma cells), HepG2 (hepatoma carcinoma cells), SGC (stomach carcinoma cells), mcf-7 (human breast cancer cells) and SCLC (small cell lung cancer cells), and can be used for preparing an anti-tumor medicine. The invention discloses a preparation method of the cortex periplocae anti-tumor effective component extractive.

Description

technical field [0001] The present invention relates to the anti-tumor effective component extract of Cortex saponica and its preparation method and application as anti-tumor medicine. Background technique [0002] Malignant tumors are one of the most serious diseases that endanger human health in the world today. Experts and scholars from all over the world are actively researching methods and drugs to treat tumors, especially the research on anti-tumor substances of traditional Chinese medicine. In recent years, there are extensive resources Traditional Chinese medicine with the advantages of multi-target, etc. has made gratifying achievements in anti-tumor. [0003] Cortex Periplocae, also known as North Wujiapi, is derived from the dried root bark of Periplocasepium Punge., a plant of the Ascariaceae family, and is a commonly used traditional Chinese medicine. This product is warm in nature, bitter in taste, poisonous, and belongs to liver, kidney and heart channels. I...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/27A61P35/00
Inventor 余江南张辉云徐希明童姗姗奚超群屈睿
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com