Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of marine chlorella polysaccharide in preparation of medicine for inhibiting tumor angiogenesis

A technology of tumor angiogenesis and marine chlorella, applied in the direction of anti-tumor drugs, drug combinations, etc., to achieve the effect of inhibiting tumor angiogenesis, proliferation and migration inhibition, and rich sources

Inactive Publication Date: 2013-04-03
DALIAN OCEAN UNIV +1
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no relevant report on the preparation of anti-tumor angiogenesis drugs from marine chlorella polysaccharides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of marine chlorella polysaccharide in preparation of medicine for inhibiting tumor angiogenesis
  • Application of marine chlorella polysaccharide in preparation of medicine for inhibiting tumor angiogenesis
  • Application of marine chlorella polysaccharide in preparation of medicine for inhibiting tumor angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0016] Referring to the literature (Anti-angiogenic activities of chitooligosaccharides, Haige Wu, Ziang Yao, Xuefang Bai, Yuguang Du, Bingcheng Lin, Carbohydrate Polymers, 73(1), 2008: 105-110), the experiment was divided into negative control group and positive control group and treatment groups (see Table 1 for details on dosing).

[0017] Negative control group: human umbilical vein endothelial cell (ECV304) culture medium 200 l;

[0018] Positive control group: at 180 l Human umbilical vein endothelial cell (ECV304) culture medium was added l The above-mentioned tumor cell culture medium, so that its concentration is 10%;

[0019] Treatment group 1: Add the extracted marine chlorella polysaccharides to the culture medium of human umbilical vein endothelial cells (ECV304) so ​​that the final concentration reaches 12.5 g?ml -1 、50 g?ml -1 、100 g?ml -1 and 200 g?ml -1 , with a final total volume of 200 l;

[0020] Treatment group 2: Add 20 l After the ...

experiment example 2

[0028] Human umbilical vein endothelial cells were divided into 1-2×10 6 Cells / ml were seeded in a 6-well plate and cultured for 24 hours. After the human umbilical vein endothelial cells grew into a single layer in the 6-well culture plate, they were scratched with a cell scraper and washed three times with PBS (pH 7.4). The marine chlorella polysaccharide solution of the present invention is added in the 3-hole culture solution, and the final concentration is respectively 12.5 g?ml -1 、50 g?ml -1 and 200 g?ml -1 At the same time, 6 wells were treated with tumor cell culture medium as an inducing factor, and observed under an inverted optical microscope after continuing to culture for 24 hours. The results were as follows: figure 1 .

[0029] Depend on figure 1 It can be seen that the tumor cell culture medium can significantly promote the migration of human umbilical vein endothelial cells; while 12.5 g?ml -1 、50 g?ml -1 and 200 g?ml -1 Chlorella polysacch...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of marine chlorella polysaccharide in the preparation of a medicine for inhibiting tumor angiogenesis. The marine chlorella polysaccharide is abundant in source of raw materials and low in cost; the effective concentration of the extracted marine chlorella polysaccharide in body fluid at the infected part is 12.5-200g / ml, the marine chlorella polysaccharide has obvious inhibition effect on the proliferation and migration of vascular endothelial cells, and therefore, the marine chlorella polysaccharide can inhibit the tumor angiogenesis and be developed to a medicine for inhibiting tumor migration, a health-care food, a food additive and the like.

Description

technical field [0001] The invention relates to an application of marine chlorella polysaccharide, in particular to an application of marine chlorella polysaccharide in preparing drugs for inhibiting tumor angiogenesis. Background technique [0002] At present, malignant tumor—cancer has become the first or second cause of human death. Whether the tumor is benign or malignant, it essentially manifests as abnormal proliferation of cells. In malignant tumors, it manifests as invasion of adjacent normal tissues and metastasis to other parts of the body through blood vessels, lymphatic vessels and body cavities, that is, tumor metastasis. Infiltration and metastasis are the main biological characteristics of malignant tumors and the main causes of death in cancer patients. Existing studies believe that the angiogenesis ability of tumors is a sign of tumor invasiveness, and there are many molecules related to tumor angiogenesis, including vascular endothelial growth factor (VEGF...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P35/00A61P35/04
Inventor 谭成玉李伟孔亮赵小霞孟繁桐
Owner DALIAN OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com