Method for producing high-efficiency biological peptide through enzymolysis and microbe fermentation

Abstract

The invention relates to a method for producing a high-efficiency biological peptide through enzymolysis and microbe fermentation, which comprises the following steps of: smashing a fermentation substrate, uniformly mixing, then adding water for material blending, and then cooking and sterilizing; when temperature is reduced to 50-55 DEG C, adding a proteolytic enzyme for hydrolyzation; after the hydrolyzation is finished, when the temperature is reduced to 28-35 DEG C, adding a mixed bacterium solution to the hydrolyzed fermentation substrate, uniformity stirring, and carrying out aerobic fermentation under the condition of constant temperature; and carrying out low-temperature drying on the fermented materials, and then smashing to obtain a high-efficiency biological peptide finished product. The fermentation substrate comprises the following materials in parts by weight: 50-60 parts of high-protein bean pulp, 10-20 parts of glutamic acid bacterial protein and 30-40 parts of dephenolized cottonseed protein, and the components are smashed and screened through a 40-mesh screen and then uniformly mixed. The mixed bacterium solution is obtained by mixing the bacterium solutions of aspergillus niger, candida tropicalis, aspergillus oryzae and bacillus subtilis according to the proportion of 1:1:1:1, and the mixed bacterium solution is inoculated into the hydrolyzed fermentation substrate in 5%-10% by weight.

Description

technical field [0001] The invention relates to a method for producing high-efficiency biological peptides through enzymolysis and microbial fermentation, and belongs to the field of microbial feed additives. Background technique [0002] The main methods of modern industrial production of small peptides include separation method, acid-base hydrolysis method, biosynthesis method, DNA recombination technology, protein enzymatic hydrolysis method and biological fermentation method. The separation method is to extract a variety of active peptides rich in animal tissues as additives, but the peptides obtained by this method have high cost, large side effects and limited sources. The acid-base hydrolysis method is to decompose the macromolecular protein into small molecular peptides through the action of strong acid and strong alkali. However, due to the corrosiveness of strong acid and strong alkali, it will bring a lot of pollution, and the degree of hydrolysis in the process o...

AI Technical Summary

Problems solved by technology

The disadvantage is that the bitterness and odor produced in the process of protein hydrolysis cannot be completely controlled, and the cost of reducing and removing the bitterness and odor in the process of hydrolysis is high; the utilization rate of protein is not high; the enzyme preparation used for hydrolysis is limited to the food industry A few microbial proteases, animal proteases and plant proteases; protein enzymatic hydrolysis method without the participation of microorganisms, most of the peptides produced have a bitter taste, poor palatability, and cannot completely remove the anti-nutritional factors in the raw materials

The biological fermentation method adopts modern biological fermentation engineering technology to enzymatically hydrolyze part of the protein in the base material into small peptides through the enzyme secreted by microorganisms during the fermentation process. It has the advantages of wide source of raw materials, mild reaction conditions, low production cost, and environmental protection. The peptide rate is lower than that of enzymatic hydrolysis

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