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Method for obtaining explants for tissue culture of bamboo

An acquisition method and explant technology, which is applied in the field of acquisition of bamboo cultivation explants, can solve the problems of low pollution rate and high induction rate, and achieve the effects of low pollution rate, strong germination ability and large number of cluster buds

Active Publication Date: 2013-03-20
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for obtaining explants of bamboo tissue culture with low pollution rate, high induction rate, low cost and simple operation for the deficiency of obtaining explants in the existing bamboo tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Acquisition of tissue culture explants of bamboo

[0024] In mid-February, select and measure the excellent green bamboo bushes and plant them in a container with a substrate. The container specification is 50 cm in diameter and 60 cm in height. The substrate in the container is 10 parts of topsoil, 3 parts of wood chaff, and decomposed organic 1 portion of fertilizer, and the row spacing of bamboo clumps is 0.7m×0.7m. After 6 months of planting, cut off the mother bamboo at a distance of 1.3m~1.5m from the ground, bury and apply 100~120g of compound fertilizer to each clump of mother bamboo, and cut off the old branches on the mother bamboo in June and February of the next year, when new shoots grow When it is too vigorous, loosen the soil around the container and remove the roots once. Cut semi-lignified branches from the mother bamboo several times every year from May to November, select branches with a diameter of 0.1-0.3 cm and 3-6 nodes, and cut off...

Embodiment 2

[0025] Embodiment 2: Diaosi bamboo tissue culture explant acquisition

[0026] In the first ten days of March, select and measure the excellent hanging silk bamboo clusters and plant them in the container with the substrate. The container specification is 80cm in diameter and 50cm in height. 2 parts of fertilizer, and the row spacing of bamboo clumps is 1.0m×1.3m. In the first ten days of September of that year, cut off the mother bamboo at a distance of 1.5m~1.8m from the ground, and apply 100~200g of compound fertilizer to each clump of mother bamboo in June, and cut off the old branches on the mother bamboo in July and March of the following year. When the bamboo shoots grow too vigorously, loosen the soil around the container and remove the roots once in March. Cut semi-lignified branches from the mother bamboo several times a year from May to November, select branches with a diameter of 0.2-0.4 cm and 4-6 nodes, and cut off the leaves. Cut every 3 nodes into a stem segm...

Embodiment 3

[0027] Embodiment 3: Acquisition of Fendan bamboo tissue culture explants

[0028] In the middle of March, select the excellent powder unit bamboo clumps that have been measured and plant them in a container with a substrate. The container specification is 70 cm in diameter and 55 cm in height. 1 part of organic fertilizer, and the row spacing of bamboo clumps is 0.8m×1.0m. After 7 months of planting, cut off the mother bamboo at a distance of 1.3m~1.5m from the ground, bury and apply 100~200g of compound fertilizer to each cluster of mother bamboo in March, and cut off the old branches on the mother bamboo in August and next March. When the new bamboo shoots grow too vigorously, loosen the soil around the container and remove the roots once in February. Cut semi-lignified branches from the mother bamboo several times a year from May to November, select branches with a diameter of 0.-0.3 cm and 3-6 nodes, and cut off the leaves. Cut every 3 nodes into a stem section, and the...

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Abstract

The invention discloses a method for obtaining explants for tissue culture of bamboo. The method comprises the steps: selecting tested fine branch stumps of bamboo groves, and planting the branch stumps in a container; cutting off the mother bamboos growing up in current year at a distance of 1.3-1.8m to the ground in August to October; applying fertilizer and trimming regularly and cutting the roots according to conditions so as to control the growth of the bamboos; clipping half-lignified branches from the mother bamboos for a plurality of times in May to November annually, cutting off the leaves and trimming the branches into stems; washing and disinfecting the stems through liquid detergent, tap water, alcohol, corrosive sublimate and sterile water; and finally trimming the stems again to obtain sterile explants for tissue culture of bamboo. Through the method, explants in large quantity with good quality and low contamination rate can be obtained, and the cluster buds have strong germination ability. The method is simple and convenient in operation and low in production cost. The method is suitable for industrialized seedling production of bambusa texlitis, bambusa chungii and dendrocalamus minor, and has good economic, social and ecological effects.

Description

technical field [0001] The invention belongs to the technical field of plant asexual propagation, and relates to a method for obtaining explants of bamboo cultivation. Background technique [0002] Bamboo plants are an important part of my country's forest resources. They have the characteristics of fast growth, early maturity, high yield, and wide application. At the same time, they have good water conservation and soil and water conservation functions. They are plants with good economic and ecological benefits. Bamboo plants rarely bloom and are not easy to bear fruit, so they basically do not use seeds to raise seedlings, but adopt the method of asexual reproduction to propagate seedlings. Seedlings mainly include methods such as cutting propagation and tissue culture. However, due to the low utilization rate of mother bamboo, the cutting propagation has great damage to the original bamboo forest resources, and the defects such as low reproduction rate are difficult to p...

Claims

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Application Information

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IPC IPC(8): A01G1/00
Inventor 郝海坤黄大勇黄开勇陈琴黄志玲李立杰徐振国
Owner GUANGXI FORESTRY RES INST
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