Cultivating method of globe artichoke
A cultivation method and artichoke technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of numerous import procedures, rapid disease spread, and high prices, and achieve stable seedling breeding methods, disease resistance and The effect of strong resistance and simple method
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[0014] Embodiment 1: (1), selection of explants: every year from October to April of the following year, samples can be taken in the field, and artichoke perennial root seedlings or tillering buds with good agronomic properties and virus-free are used as explants;
[0015] (2) Disinfection of explants: Rinse the explant materials collected from the field with running water, cut off the large leaves first, and then use a blade to cut off the leaves and tissues around the shoot tip, and take 3-4cm stems After cleaning the tip, transfer it to the sterile room; on the ultra-clean workbench, first treat it with 75% alcohol for 10 seconds, wash it for 2 to 3 times, then treat it with 0.1% mercury liter for 8 to 10 minutes, and rinse it with sterile water for 4 minutes. After ~5 times, put it on sterile filter paper to absorb the water and set aside;
[0016] (3) Induction of clustered buds: Cut the sterilized shoot tip into 0.5 cm parts and insert into bud induction medium MS+1 mg / L...
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