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Enterobacter aerogenes specific PCR (polymerase chain reaction) detection primer

A technology for the detection of Enterobacter aerogenes and primers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve high sensitivity, avoid cumbersome operations, and be easy to determine

Active Publication Date: 2014-05-14
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Abstract
  • Description
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Problems solved by technology

However, through the literature search of the prior art, it is found that there is no report on the detection of Enterobacter aerogenes by PCR technology.

Method used

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  • Enterobacter aerogenes specific PCR (polymerase chain reaction) detection primer
  • Enterobacter aerogenes specific PCR (polymerase chain reaction) detection primer
  • Enterobacter aerogenes specific PCR (polymerase chain reaction) detection primer

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Embodiment Construction

[0024] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental method that does not indicate specific condition in the embodiment, generally according to conventional conditions such as molecular cloning such as Sambrook: the condition described in the laboratory handbook (New York: Cold Spring Habor Laboratory Press, 1989), or according to manufacturer's suggestion condition.

[0025] Step 1: Design primer pairs for specific amplification of Enterobacter aerogenes

[0026] The primer sequence is: F: 5'-GTAACCGGTGAAACCGAAAGC-3'

[0027] R: 5'-GATGCCGCCTTCGTAGTGGAAATGG-3';

[0028] Step 2, DNA template preparation

[0029] Inoculate Enterobacter aerogenes in 5ml of nutrient broth liquid medium, shake and culture in a constant temperature shaker at 35°C for 12 hours to...

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Abstract

The invention relates to a primer for detection, specifically relates to a PCR (polymerase chain reaction) detection primer for detecting enterobacter aerogenes and belongs to the technical field of biological detection. The enterobacter aerogenes specific PCR detection primer comprises a forward primer and a reverse primer, wherein the forward primer F is 5'-GTAACCGGTGAAACCGAAAGC-3'; and the reverse primer R is 5'-GATGCCGCCTTCGTAGTGGAAATGG-3'. A detection method for detecting the enterobacter aerogenes, disclosed by the invention, has the advantages that the required detection time is short, the specificity is strong and the sensitivity is high. The PCR detection primer disclosed by the invention can avoid the shortcomings of trivial operation, long time, low accuracy, low detection rate and the like of a traditional identification method.

Description

technical field [0001] The invention relates to a primer for detection, in particular to a primer for PCR detection of Enterobacter aerogenes, belonging to the technical field of biological detection. Background technique [0002] Enterobacter aerogenes (Enterobacter aerogenes) is a Gram-negative bacterium that mainly exists in the intestinal tract of humans and animals, and can also exist in water, soil and spoilage. It is also an important conditional pathogen. Infection often occurs when the host's state changes or bacteria enter a site other than the gut. In recent years, due to the widespread clinical use of extended-spectrum antibacterial drugs such as third-generation and fourth-generation cephalosporins and carbapenems, the number of drug-resistant strains of this bacteria has increased significantly, and some strains have shown multiple resistance. medicine. Multi-drug resistance complicates the clinical treatment of diseases caused by the bacteria. One of the ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/10C12N15/11
CPCY02A50/30
Inventor 陈雪峰杨国梁王军毅高强张宇飞黄雪娜
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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