Method for determining retinal cell death process

Abstract

The invention belongs to the field of molecular biology analysis and detection, and discloses a method for determining a retinal cell death process. Firstly, a total protein sample except nucleoprotein is separated from a diseased retinal tissue, and antigen protein which has immunoreaction with an anti-phosphorylated extracellular signal-regulated kinase 1 / 2 (P-ERK1 / 2) specific antibody in the extranuclear total protein sample is detected by a western blot method for quantitative analysis; comparison is performed with a retinal cell death process detected by immunohistochemistry so as to determine the retinal cell death process. The invention can determine a cell death process in genetic retinal degeneration process according to the level change of the phosphorylated extracellular signal-regulated kinase 1, 2, and can provide critical and important information for the illustration of genetic disease tissue cell death and the discussion of prevention and treatment means.

Description

technical field [0001] The invention belongs to the field of molecular biology analysis and detection, and relates to a method for judging the death process of retinal cells. Background technique [0002] Genetic diseases have plagued human beings for a long time. Hereditary retinal degeneration is a process in which retinal cells die rapidly, leading to blindness rapidly. A large amount of research work has been carried out and is ongoing in the world, but the molecular biological mechanism of this disease, especially the process of rapid death of retinal cells, is not well understood; in order to better understand and prevent these diseases, a large number of Advanced molecular biology techniques are being used to study these diseases. Extracellular signal-regulated kinases (ERK1, ERK2) are important members of some signal transduction systems that function in the process of cell differentiation and proliferation, although there have been many materials proving that extra...

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Problems solved by technology

Extracellular signal-regulated kinases (ERK1, ERK2) are important members of some signal transduction systems that function in the process of cell differentiation and proliferation, although there have been a lot of materials to prove that extracellular signal-regulated kinases 1 and 2 (ERK1 / 2) play a role in The process of apoptosis plays a key role in signal transmission and regulation of gene expression; however, no studies have established the relationship between the level of phosphorylated extracellular signal-regulated kinase 1, 2 (P-ERK1 / 2) and the process of inherited retinal degeneration. The correlation of abnormal cell death process, and there is no relevant report on the level of phosphorylated extracellular signal-regulated kinase 1, 2 (P-ERK1 / 2) as the basis for judging the cell death process in the process of hereditary retinal degeneration

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