High-throughput method for extracting carotene in biological sample

Abstract

The invention relates to a high-throughput method for extracting carotene in a biological sample. The method comprises the following steps of: providing oscillating and crushing power through a mechanical oscillator fixed with an oscillating and crushing tube rack, filling the biological sample, an oscillating and crushing ball and an organic solvent in the oscillating and crushing tube, and locking the sealed oscillating and crushing tube on the oscillator tube rack; and realizing grinding crushing of tissues or cells of the biological sample and dissolving and extracting of a carotene sample by utilizing collision friction of the oscillating and crushing ball and osmosis dissolution of the organic solvent in a tube at a certain oscillating rotating speed or frequency. Compared with the prior art, the method has the advantages of sample treatment flux, simple process, high working efficiency, low experimental system error, fewer required samples and solvents, low labor intensity, insulation of solvent volatilization and the like; and according to the method, the sample treatment flux and the carotene extraction efficiency can be effectively improved in the process of industrial microorganism, animal and plant high-quality variety breeding and process optimization.

Description

technical field [0001] The present invention relates to the technical field of carotenoid production and research, specifically, it is a kind of closed crushing tube assembled with organic solvents, oscillating broken beads and samples to be extracted by using an oscillating frame fixed on a high-speed oscillator. oscillate simultaneously. With the help of the collision milling effect of the oscillating crushing beads and the extraction effect of the organic solvent, the carotenoids contained in the cells in each crushing tube are dissolved and extracted at the same time, so as to realize the high-throughput and rapid extraction of carotenoids from the analysis samples . Background technique [0002] Carotene was first isolated from carrots by Wackenroder in 1831, the molecular formula of β-carotene was determined by Willstather in 1906, and the structural formula was determined by Zechmeister et al. in 1928-1932. Carotene is an orange-colored photosynthetic pigment that w...

AI Technical Summary

Problems solved by technology

The main characteristics of these technologies are: the required devices are large and expensive; there are many materials to be processed, but each time the loss and the required sample volume are large; The sample is extracted repeatedly); the amount of solvent required is relatively large; the extraction process often requires heat treatment, and the operating cost is high; each device can only process a single sample each time

For the bacterial strain breeding of excellent industrial production strains and the fermentation optimization process of the optimal process technology, due to the extremely large (from tens to hundreds of samples / time) of the required extraction and analysis samples, the previous literature patents The publicly reported technology has shortcomings such as time-consuming, labor-intensive, energy-consuming, labor-intensive, low processing throughput, large sample demand and loss in the extraction process, and is not suitable for the simultaneous processing of a large number of samples and the needs of sample analysis.

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