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Application of obese receptor (OB) as goose fat character genetic marker

A genetic marker and gene technology, applied in the direction of genetic engineering, plant genetic improvement, application, etc., can solve the problems of no goose OB gene research report, fewer chickens, etc., and achieve significant application value and economic benefits.

Inactive Publication Date: 2013-02-06
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on the DNA genetic markers, genetic diversity and related functional genes of goose compared with chickens, and there is no research report on goose OB gene

Method used

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  • Application of obese receptor (OB) as goose fat character genetic marker
  • Application of obese receptor (OB) as goose fat character genetic marker
  • Application of obese receptor (OB) as goose fat character genetic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 SNP screening of goose related genes

[0027] 150 healthy young Xupu geese and 150 Sichuan white geese of the same age were selected for feeding experiment. All test geese were slaughtered at 84 days of age, and the measurement and calculation were carried out in accordance with the "Poultry Production Performance Terminology and Statistical Methods of Measurement" in the "Technical Manual for the Investigation of Livestock and Poultry Genetic Resources". Use electronic scales to measure carcass weight, eviscerated weight, abdominal fat weight, and sebum thickness with calipers. Calculate the carcass fat trait ratio index.

[0028] After 12 hours of fasting for Xupu goose and Sichuan white goose that are about to be slaughtered, use a disposable medical syringe (2.5ml) to collect blood from the underwing vein, 1ml / only in a 5ml centrifuge tube, anticoagulate with 75% ethanol, refrigerator at 4℃ Keep refrigerated for later use.

[0029] Six samples were selected fr...

Embodiment 2

[0043] Example 2 Verification of SNP related to abdominal fat rate in production practice

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Abstract

The invention discloses an obese receptor (OB) gene and application thereof as a goose fat character genetic marker. Genetic markers in the OB gene relevant to goose abdominal fat weight and abdominal fat rate are determined, and the polymorphism in the OB gene in a nucleic acid sample obtained from a goose is determined and causes that the coded amino acid changes and is relevant to the abdominal fat weight and the abdominal fat rate. According to the single nucleotide polymorphism of the OB gene, animals containing the OB genes in a genome DNA are subjected to fatty breeding, and protogene types reducing the abdominal fat weight and abdominal fat rate of the goose are screened, thus the OB gene has important application value and economic benefit.

Description

Technical field [0001] The present invention relates to the field of molecular biology technology and breeding of goose, in particular to the determination of the presence of genetic markers related to abdominal fat weight and abdominal fat rate, and more specifically, to the determination of OB genes showing these characteristics The difference, more specifically, is the determination of polymorphisms in the OB gene, that is, the single nucleotide polymorphism of the goose OB (obese) gene related to abdominal fat weight and abdominal fat rate (Single nucleotide polymorphism, abbreviated as SNP) exists. Background technique [0002] The development of modern poultry genetic breeding theory and application has greatly increased the growth rate and feed efficiency of meat poultry, especially broiler chickens and ducks, and the production efficiency is gradually adapted to factory production. But at the same time, it is easy to cause a large amount of carcass abdominal fat and subc...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12Q1/68A01K67/027
Inventor 曲湘勇蒋隽何俊贺长青
Owner HUNAN AGRICULTURAL UNIV
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